Detection of β-1-4-galactan in compression wood of Sitka Spruce (Picea sitchensis (Bong.) Carrière) by immunofluorescence.

Hapca, Adrian I.; Altaner, Clemens; Knox, J. Paul; Jarvis, Michael C.

Abstract

β-1-4-Galactan has been detected in Sitka spruce [Picea sitchensis (Bong.) Carrière] by an immunofluorescence procedure. The anti-β-1-4-galactan monoclonal antibody LM5 was found to bind to compression wood (CW) tissue as identified by cell morphology features. In cross-sections, year rings with very fine bands of galactan-containing tissue were found. Cells in these bands expressed no morphological CW features. This finding was interpreted to imply that β-1-4-galactan synthesis is one of the first physiological reactions of CW formation. In cases where the CW character was pronounced, galactan was exclusively located in the outer cell-wall layers and was absent from the majority of the inner S2 layer.

Citation

Hapca, A. I., Altaner, C., Knox, J. P., & Jarvis, M. C. (2007). Detection of β-1-4-galactan in compression wood of Sitka Spruce (Picea sitchensis (Bong.) Carrière) by immunofluorescence. Holzforschung, 61(3), 311-316. https://doi.org/10.1515/HF.2007.049

• Journal Article Type: Article
• Publication Date: May 1, 2007
• Deposit Date: Feb 18, 2008
• Print ISSN: 0018-3830
• Electronic ISSN: 1437-434X
• Publisher: De Gruyter
• Peer Reviewed: Peer Reviewed
• Volume: 61
• Issue: 3
• Pages: 311-316
• DOI: https://doi.org/10.1515/HF.2007.049
• Keywords: Sitka Spruce; Compression wood; Galactan; Detection; Immunofluorescence
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/1964
• Publisher URL: http://dx.doi.org/10.1515/HF.2007.049