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Improved protection against avian influenza H5N1 virus by a single vaccination with virus-like particles in skin using microneedles

Song, Jae-Min; Kim, Yeu-Chun; Barlow, Peter G.; Hossain, M. Jaber; Park, Kyoung-Mi; Donis, Ruben O.; Prausnitz, Mark R.; Compans, Richard W.; Kang, Sang-Moo


Jae-Min Song

Yeu-Chun Kim

M. Jaber Hossain

Kyoung-Mi Park

Ruben O. Donis

Mark R. Prausnitz

Richard W. Compans

Sang-Moo Kang


To develop a more effective vaccination method against H5N1 virus, we investigated the immunogenicity and protective efficacy after skin vaccination using microneedles coated with influenza virus-like particles containing hemagglutinin derived from A/Vietnam/1203/04 H5N1 virus (H5 VLPs). A single microneedle vaccination of mice with H5 VLPs induced increased levels of antibodies and provided complete protection against lethal challenge without apparent disease symptoms. In contrast, intramuscular injection with the same vaccine dose showed low levels of antibodies and provided only partial protection accompanied by severe body weight loss. Post-challenge analysis suggested that improved protection was associated with lower lung viral titers and enhanced generation of recall antibody secreting cells by microneedle vaccination. Thus, this study provides evidence that skin delivery of H5 VLP vaccines using microneedles designed for self-administration induces improved protection compared to conventional intramuscular immunization.

Journal Article Type Other
Acceptance Date Sep 1, 2010
Online Publication Date Sep 21, 2010
Publication Date 2010-11
Deposit Date Mar 27, 2012
Journal Antiviral Research
Print ISSN 0166-3542
Electronic ISSN 0166-3542
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 88
Issue 2
Pages 244-247
Keywords H5N1; Pandemic vaccine; Single dose; Skin vaccination; Microneedles
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