Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe

Duprey, Jean-Louis H A; Zhao, Zheng-yun; Bassani, Dario M; Manchester, Jack; Vyle, Joseph S; Tucker, James H R

Abstract

A fluorescent DNA probe containing an anthracene group attached via an anucleosidic linker can identify all four DNA bases at a single site as well as the epigenetic modification C/5-MeCvia a hybridisation sensing assay.

Citation

Duprey, J. H. A., Zhao, Z., Bassani, D. M., Manchester, J., Vyle, J. S., & Tucker, J. H. R. (2011). Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe. Chemical Communications, 47, 6629. https://doi.org/10.1039/c1cc11205h

• Journal Article Type: Article
• Publication Date: 2011
• Deposit Date: Apr 10, 2014
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 47
• Pages: 6629
• DOI: https://doi.org/10.1039/c1cc11205h
• Keywords: DNA base variation; cytosine methylation; highly sensitive fluorescent probe
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/6770
• Publisher URL: http://dx.doi.org/10.1039/c1cc11205h