Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

Zhao, Zheng-yun; San, Marie; Duprey, Jean-Louis H A; Arrand, John R; Vyle, Joseph S; Tucker, James H R

Abstract

Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe–target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base–pair as either matching or mismatching.

Citation

Zhao, Z.-Y., San, M., Duprey, J.-L. H. A., Arrand, J. R., Vyle, J. S., & Tucker, J. H. R. (2012). Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe. Bioorganic and Medicinal Chemistry Letters, 22(1), 129-132. https://doi.org/10.1016/j.bmcl.2011.11.053

• Journal Article Type: Article
• Publication Date: 2012-01
• Deposit Date: Apr 10, 2014
• Print ISSN: 0960-894X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 1
• Pages: 129-132
• DOI: https://doi.org/10.1016/j.bmcl.2011.11.053
• Keywords: DNA; SNP; Fluorescence; Sensing; Nucleic acids
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/6771
• Publisher URL: http://dx.doi.org/10.1016/j.bmcl.2011.11.053