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Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

Zhao, Zheng-yun; San, Marie; Duprey, Jean-Louis H A; Arrand, John R; Vyle, Joseph S; Tucker, James H R

Authors

Zheng-yun Zhao

Marie San

Jean-Louis H A Duprey

John R Arrand

Joseph S Vyle

James H R Tucker



Abstract

Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe–target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base–pair as either matching or mismatching.

Journal Article Type Article
Publication Date 2012-01
Deposit Date Apr 10, 2014
Print ISSN 0960-894X
Electronic ISSN 1464-3405
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 22
Issue 1
Pages 129-132
DOI https://doi.org/10.1016/j.bmcl.2011.11.053
Keywords DNA; SNP; Fluorescence; Sensing; Nucleic acids;
Public URL http://researchrepository.napier.ac.uk/id/eprint/6771
Publisher URL http://dx.doi.org/10.1016/j.bmcl.2011.11.053



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