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GABAA receptor chloride channels are involved in the neuroprotective role of GABA following oxygen and glucose deprivation in the rat cerebral cortex but not in the hippocampus

Darlison, Mark G.; Dos-Anjos, Severiano; Llorente, Irene L.; Llorente, Irene L; Perez-Rodriguez, Diego; Mart�nez-Villayandre, Beatriz; Dos Anjos, Severiano; Darlison, Mark G; Poole, Amy V.; Fern�ndez-L�pez, Arsenio

Authors

Mark G. Darlison

Severiano Dos-Anjos

Irene L. Llorente

Irene L Llorente

Diego Perez-Rodriguez

Beatriz Mart�nez-Villayandre

Severiano Dos Anjos

Mark G Darlison

Arsenio Fern�ndez-L�pez



Abstract

Assays on "ex vivo" sections of rat hippocampus and rat cerebral cortex, subjected to oxygen and glucose deprivation (OGD) and a three-hour reperfusion-like (RL) recovery, were performed in the presence of either GABA or the GABA(A) receptor binding site antagonist, bicuculline. Lactate dehydrogenase (LDH) and propidium iodide were used to quantify cell mortality. We also measured, using real-time quantitative polymerase chain reaction (qPCR), the early transcriptional response of a number of genes of the glutamatergic and GABAergic systems. Specifically, glial pre- and post-synaptic glutamatergic transporters (namely GLAST1a, EAAC-1, GLT-1 and VGLUT1), three GABAA receptor subunits (α1, β2 and γ2), and the GABAergic presynaptic marker, glutamic acid decarboxylase (GAD65), were studied. Mortality assays revealed that GABAA receptor chloride channels play an important role in the neuroprotective effect of GABA in the cerebral cortex, but have a much smaller effect in the hippocampus. We also found that GABA reverses the OGD-dependent decrease in GABA(A) receptor transcript levels, as well as mRNA levels of the membrane and vesicular glutamate transporter genes. Based on the markers used, we conclude that OGD results in differential responses in the GABAergic presynaptic and postsynaptic systems.

Citation

Darlison, M. G., Dos-Anjos, S., Llorente, I. L., Llorente, I. L., Perez-Rodriguez, D., Martínez-Villayandre, B., …Fernández-López, A. (2013). GABAA receptor chloride channels are involved in the neuroprotective role of GABA following oxygen and glucose deprivation in the rat cerebral cortex but not in the hippocampus. Brain Research, 1533, 141-151. https://doi.org/10.1016/j.brainres.2013.08.024

Journal Article Type Article
Publication Date 2013-10
Deposit Date Mar 13, 2014
Print ISSN 0006-8993
Electronic ISSN 1872-6240
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1533
Pages 141-151
DOI https://doi.org/10.1016/j.brainres.2013.08.024
Keywords Developmental Biology; General Neuroscience; Molecular Biology; Clinical Neurology
Public URL http://researchrepository.napier.ac.uk/id/eprint/6627
Publisher URL http://dx.doi.org/10.1016/j.brainres.2013.08.024