FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression

Ikpegbu, Ekele; Basta, Lena; Clements, Dylan N.; Fleming, Robert; Vincent, Tonia L.; Buttle, David J.; Pitsillides, Andrew A.; Staines, Katherine A.; Farquharson, Colin

Abstract

E11/podoplanin is critical in the early stages of osteoblast-to-osteocyte transitions (osteocytogenesis) however the upstream events which regulate E11 expression are unknown. The aim of this study was to examine the effects of FGF-2 on E11-mediated osteocytogenesis and to reveal the nature of the underlying signaling pathways regulating this process. Exposure of MC3T3 osteoblast-like cells and murine primary osteoblasts to FGF-2 (10ng/ml) increased E11 mRNA and protein expression (P

Citation

Ikpegbu, E., Basta, L., Clements, D. N., Fleming, R., Vincent, T. L., Buttle, D. J., Pitsillides, A. A., Staines, K. A., & Farquharson, C. (2018). FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression. Journal of Cellular Physiology, 233(7), 5334-5347. https://doi.org/10.1002/jcp.26345

• Journal Article Type: Article
• Acceptance Date: Nov 29, 2017
• Online Publication Date: Dec 7, 2017
• Publication Date: Jan 23, 2018
• Deposit Date: Nov 30, 2017
• Publicly Available Date: Feb 13, 2018
• Journal: Journal of Cellular Physiology
• Print ISSN: 0021-9541
• Electronic ISSN: 1097-4652
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 233
• Issue: 7
• Pages: 5334-5347
• DOI: https://doi.org/10.1002/jcp.26345
• Keywords: Osteocytes, osteoblasts, E11/podoplanin, FGF-2, osteocytogenesis.
• Public URL: http://researchrepository.napier.ac.uk/Output/1015193
• Contract Date: Feb 13, 2018