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Efficacy of two Chlamydia abortus subcellular vaccines in a pregnant ewe challenge model for ovine enzootic abortion (2021)
Journal Article
Livingstone, M., Ranjan Wattegedera, S., Palarea-Albaladejo, J., Aitchison, K., Corbett, C., Sait, M., Wilson, K., Chianini, F., Silvia Rocchi, M., Wheelhouse, N., Entrican, G., & Longbottom, D. (2021). Efficacy of two Chlamydia abortus subcellular vaccines in a pregnant ewe challenge model for ovine enzootic abortion. Vaccines, 9(8), Article 898. https://doi.org/10.3390/vaccines9080898

Chlamydia abortus, the aetiological agent of enzootic abortion of ewes, is a major cause of reproductive loss in small ruminants worldwide, accounting for significant economic losses to the farming industry. Disease can be managed through the use of... Read More about Efficacy of two Chlamydia abortus subcellular vaccines in a pregnant ewe challenge model for ovine enzootic abortion.

Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells (2021)
Journal Article
Brown, M., Rae, M., & Wheelhouse, N. (2021). Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells. Reproduction and Fertility, 2(1), 9-11. https://doi.org/10.1530/RAF-20-0069

Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the UK, with over 200,000 positive diagnoses annually. The infection is thought to cause reproductive complications including problems in conceiving a pregnancy th... Read More about Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells.

Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania (2020)
Journal Article
Theonest, N. O., Carter, R. W., Kasagama, E., Keyyu, J. D., Shirima, G. M., Tarimo, R., Thomas, K. M., Wheelhouse, N., Maro, V. P., Haydon, D. T., Buza, J. J., Allan, K. J., & Halliday, J. E. (2021). Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania. Veterinary Medicine and Science, 7(3), 960-967. https://doi.org/10.1002/vms3.401

Coxiella burnetii is an obligate intracellular bacterium that causes Q fever, a zoonotic disease of public health importance. In northern Tanzania, Q fever is a known cause of human febrile illness, but little is known about its distribution in anima... Read More about Molecular detection of Coxiella burnetii infection in small mammals from Moshi Rural and Urban Districts, northern Tanzania.

Defining immune correlates during latent and active chlamydial infection in sheep (2020)
Journal Article
Ranjan Wattegedera, S., Livingstone, M., Maley, S., Rocchi, M., Lee, S., Pang, Y., Wheelhouse, N. M., Aitchison, K., Palarea-Albaladejo, J., Buxton, D., Longbottom, D., & Entrican, G. (2020). Defining immune correlates during latent and active chlamydial infection in sheep. Veterinary Research, 51, Article 75 (2020). https://doi.org/10.1186/s13567-020-00798-6

Ovine enzootic abortion (OEA) caused by the obligate intracellular bacterial pathogen Chlamydia abortus (C. abortus), is an endemic disease in most sheep-rearing countries worldwide. Following infection, C. abortus establishes a complex host–pathogen... Read More about Defining immune correlates during latent and active chlamydial infection in sheep.

Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning (2019)
Journal Article
Longbottom, D., Livingstone, M., Aitchison, K. D., Imrie, L., Manson, E., Wheelhouse, N., & Inglis, N. F. (2019). Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning. PLOS ONE, 14(10), Article e0224070. https://doi.org/10.1371/journal.pone.0224070

Data are presented on the identification and partial characterisation of proteins comprising the chlamydial outer membrane complex (COMC) fraction of Chlamydia abortus (C. abortus)—the aetiological agent of ovine enzootic abortion. Inoculation with t... Read More about Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning.

Understanding the attitudes and acceptability of extra-genital Chlamydia testing in young women: evaluation of a feasibility study (2019)
Journal Article
Brown, S., Paterson, C., Dougall, N., Cameron, S., & Wheelhouse, N. (2019). Understanding the attitudes and acceptability of extra-genital Chlamydia testing in young women: evaluation of a feasibility study. BMC Public Health, 19(1), Article 992. https://doi.org/10.1186/s12889-019-7313-0

Background
Chlamydia trachomatis (C. trachomatis) is the most common bacterial sexually transmitted infection in the UK. Recent studies suggest that in addition to the genital tract, C. trachomatis is found in the throat and rectum, suggesting the n... Read More about Understanding the attitudes and acceptability of extra-genital Chlamydia testing in young women: evaluation of a feasibility study.

Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential. (2019)
Journal Article
Smith, A., Hearn, J., Taylor, C., Wheelhouse, N., Kaczmarek, M., Moorhouse, E., & Singleton, I. (2019). Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential. International Journal of Food Microbiology, 299, 23-32. https://doi.org/10.1016/j.ijfoodmicro.2019.03.013

Listeria monocytogenes is sporadically detected on a range of ready to eat fresh produce lines, such as spinach and rocket, and is a threat to public health. However, little is known about the diversity of L. monocytogenes present on fresh produce an... Read More about Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential..

Current perspectives on the transmission of Q fever: Highlighting the need for a systematic molecular approach for a neglected disease in Africa. (2019)
Journal Article
Salifu, S. P., Bukari, A.-R. A., Frangoulidis, D., & Wheelhouse, N. (2019). Current perspectives on the transmission of Q fever: Highlighting the need for a systematic molecular approach for a neglected disease in Africa. Acta tropica, 193, 99-105. https://doi.org/10.1016/j.actatropica.2019.02.032

Q fever is a bacterial worldwide zoonosis (except New Zealand) caused by the Gram-negative obligate intracellular bacterium Coxiella burnetii (C. burnetii). The bacterium has a large host range including arthropods, wildlife and companion animals and... Read More about Current perspectives on the transmission of Q fever: Highlighting the need for a systematic molecular approach for a neglected disease in Africa..

Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction (2019)
Journal Article
Wattegedera, S. R., Doull, L. E., Goncheva, M. I., Wheelhouse, N., Watson, D. M., Pearce, J., Benavides, J., Palarea-Albaladejo, J., Mcinnes, C. J., Ballingall, K., & Entrican, G. (2019). Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction. Frontiers in Immunology, 9, https://doi.org/10.3389/fimmu.2018.03025

Successful mammalian pregnancies are a result of complex physiological, endocrinological and immunological processes that combine to create an environment where the mother is tolerant to the semi-allogeneic fetus. Our knowledge of the mechanisms that... Read More about Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction.

Ferlavirus-related deaths in a collection of viperid snakes (2018)
Journal Article
Flach, E. J., Dagleish, M. P., Feltrer, Y., Gill, I. S., Marschang, R. E., Masters, N., Orós, J., Pocknell, A., Rendle, M. E., Strike, T., Tapley, B., & Wheelhouse, N. M. (2018). Ferlavirus-related deaths in a collection of viperid snakes. Journal of Zoo and Wildlife Medicine, 49(4), 983-995. https://doi.org/10.1638/2017-0223.1

Between June and October 2013 26 snakes of six viperid species kept in two adjoining rooms died (n = 16) or were euthanized on medical (1) or welfare grounds (9). Two were from the main zoo collection, but the other 24 had been imported and quarantin... Read More about Ferlavirus-related deaths in a collection of viperid snakes.

Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment (2018)
Journal Article
Ahmad, S. F., Brown, J. K., Campbell, L. L., Koscielniak, M., Oliver, C., Wheelhouse, N., Entrican, G., McFee, S., Wills, G. S., McClure, M. O., Horner, P. J., Gaikoumelou, S., Lee, K. F., Critchley, H. O., Duncan, W. C., & Horne, A. W. (2018). Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment. EBioMedicine, 29, 159-165. https://doi.org/10.1016/j.ebiom.2018.02.020

Tubal ectopic pregnancies are a leading cause of global maternal morbidity and mortality. Previous infection with Chlamydia trachomatis is a major risk factor for tubal embryo implantation but the biological mechanism behind this association is uncle... Read More about Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment.

Control of Antimicrobial Resistance Requires an Ethical Approach (2017)
Journal Article
Parsonage, B., Hagglund, P. K., Keogh, L., Wheelhouse, N., Brown, R. E., & Dancer, S. J. (2017). Control of Antimicrobial Resistance Requires an Ethical Approach. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.02124

Ethical behavior encompasses actions that benefit both self and society. This means that tackling antimicrobial resistance (AMR) becomes an ethical obligation, because the prospect of declining anti-infectives affects everyone. Without preventive act... Read More about Control of Antimicrobial Resistance Requires an Ethical Approach.

Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain (2017)
Journal Article
Velasova, M., Damaso, A., Prakashbabu, B. C., Gibbons, J., Wheelhouse, N., Longbottom, D., Van Winden, S., Green, M., & Guitian, J. (2017). Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain. Journal of Dairy Science, 100(11), 9215-9233. https://doi.org/10.3168/jds.2016-11863

To implement appropriate and effective disease control programs at national level, up-to-date and unbiased information on disease frequency is needed. The aim of this study was to estimate the prevalence of selected endemic infectious diseases in th... Read More about Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain.

Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release (2017)
Journal Article
Giakoumelou, S., Wheelhouse, N., Brown, J., Wade, J., Simitsidellis, I., Gibson, D., Saunders, P. T. K., Horner, P., Entrican, G., Howie, S. E. M., & Horne, A. W. (2017). Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release. Scientific Reports, 7(1), Article 2001. https://doi.org/10.1038/s41598-017-02223-z

Miscarriage affects ~20% of pregnancies and 30 maternal infections account for ~15% of early miscarriages. Chlamydia trachomatis (Ct) has been associated with miscarriage but the underlying mechanisms are unknown. Successful implantation requires end... Read More about Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release.

Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS (2017)
Journal Article
Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., Wattegedera, S., Wilson, K., Sait, M., Siarkou, V., Vretou, E., Entrican, G., Dagleish, M., & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLOS ONE, 12(5), Article e0177653. https://doi.org/10.1371/journal.pone.0177653

This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the thr... Read More about Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila (2016)
Journal Article
Wheelhouse, N., Flockhart, A., Aitchison, K., Livingstone, M., Finlayson, J., Flachon, V., Sellal, E., Dagleish, M. P., & Longbottom, D. (2016). Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila. Scientific Reports, 6, 37150. https://doi.org/10.1038/srep37150

Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondr... Read More about Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila.

Challenges and priorities for modelling livestock health and pathogens in the context of climate change (2016)
Journal Article
Özkan, Ş., Vitali, A., Lacetera, N., Amon, B., Bannink, A., Bartley, D. J., Blanco-Penedo, I., de Haas, Y., Dufrasne, I., Elliott, J., Eory, V., Fox, N. J., Garnsworthy, P. C., Gengler, N., Hammami, H., Kyriazakis, I., Leclère, D., Lessire, F., Macleod, M., Robinson, T. P., …Kipling, R. P. (2016). Challenges and priorities for modelling livestock health and pathogens in the context of climate change. Environmental Research, 151, 130-144. https://doi.org/10.1016/j.envres.2016.07.033

Climate change has the potential to impair livestock health, with consequences for animal welfare, productivity, greenhouse gas emissions, and human livelihoods and health. Modelling has an important role in assessing the impacts of climate change on... Read More about Challenges and priorities for modelling livestock health and pathogens in the context of climate change.

Ovine trophoblasts express cathelicidin host defence peptide in response to infection (2016)
Journal Article
Coyle, C., Wheelhouse, N., Jacques, M., Longbottom, D., Svoboda, P., Pohl, J., Colin Duncan, W., Rae, M. T., & Barlow, P. G. (2016). Ovine trophoblasts express cathelicidin host defence peptide in response to infection. Journal of Reproductive Immunology, 117, 10-16. https://doi.org/10.1016/j.jri.2016.06.006

Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placental trophoblast in ovine host defence remains poorly understood. This study... Read More about Ovine trophoblasts express cathelicidin host defence peptide in response to infection.

MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells (2016)
Journal Article
Storrie, S., Longbottom, D., Barlow, P. G., & Wheelhouse, N. (2016). MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells. PLOS ONE, 11(3), Article e0152193. https://doi.org/10.1371/journal.pone.0152193

Background
Waddlia chondrophila (W. chondrophila) is an emerging agent of respiratory and reproductive disease in humans and cattle. The organism is a member of the order Chlamydiales, and shares many similarities at the genome level and in growth s... Read More about MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells.