Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release

Giakoumelou, Sevi; Wheelhouse, Nick; Brown, Jeremy; Wade, Jean; Simitsidellis, Ioannis; Gibson, Douglas; Saunders, Philippa T. K.; Horner, Patrick; Entrican, Gary; Howie, Sarah E. M.; Horne, Andrew W.

Abstract

Miscarriage affects ~20% of pregnancies and 30 maternal infections account for ~15% of early miscarriages. Chlamydia trachomatis (Ct) has been associated with miscarriage but the underlying mechanisms are unknown. Successful implantation requires endometrial stromal cell (ESC) decidualisation. Maintenance of pregnancy requires angiogenesis, establishment of the correct cellular milieu and trophoblast invasion, all of which involve the action of chemokines. Our objective was to determine whether Ct infection impacts upon ESC decidualisation and chemokine secretion. Human primary ESC were decidualised in-vitro, infected with Ct serovar E, and changes in expression of genes of interest were measured using RT-PCR, proteomic array and ELISA. We demonstrate for the first time that Ct can infect and proliferate in ESC. Expression of the decidualisation marker prolactin was decreased in Ct-infected ESC at both mRNA and protein levels. Ct infection altered the chemokine profile of decidualised ESC as shown by proteomic array. Chemokines CXCL12 and CXCL16, important for trophoblast invasion, were analysed further and expression was reduced in infected decidualised cells at mRNA and protein levels. Our data indicate that Ct infection of ESC impairs decidualisation and alters chemokine release. These findings at least partially explain how Ct infection could result in adverse pregnancy outcomes.

Citation

Giakoumelou, S., Wheelhouse, N., Brown, J., Wade, J., Simitsidellis, I., Gibson, D., Saunders, P. T. K., Horner, P., Entrican, G., Howie, S. E. M., & Horne, A. W. (2017). Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release. Scientific Reports, 7(1), Article 2001. https://doi.org/10.1038/s41598-017-02223-z

• Journal Article Type: Article
• Acceptance Date: Apr 7, 2017
• Online Publication Date: May 17, 2017
• Publication Date: 2017-12
• Deposit Date: Apr 11, 2017
• Publicly Available Date: Jul 26, 2019
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 1
• Article Number: 2001
• DOI: https://doi.org/10.1038/s41598-017-02223-z
• Keywords: Infection, Chlamydia trachomatis, miscarraige, endometrial stromal cell (ESC) decidualisation, chemokines,
• Public URL: http://researchrepository.napier.ac.uk/Output/827253
• Contract Date: Apr 11, 2017