The conserved metalloprotease invadolysin localizes to the surface of lipid droplets

Cobbe, Neville; Marshall, Kathryn M.; Rao, SHubuha Gururaja; Chang, Ching-Wen; Di Cara, Francesca; Duca, Edward; Vass, Sharron; Kassan, Adam; Heck, Margarete M.S.

Abstract

Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent detection of human invadolysin in cultured cells reveals the protein to be associated with the surface of lipid droplets. By means of subcellular fractionation, we have independently confirmed the association of invadolysin with lipid droplets. We thus identify invadolysin as the first metalloprotease located on these dynamic organelles. In addition, analysis of larval fat-body morphological appearance and triglyceride levels in the Drosophila invadolysin mutant suggests that invadolysin plays a role in lipid storage or metabolism.

• Journal Article Type: Article
• Acceptance Date: Jun 25, 2009
• Online Publication Date: Aug 25, 2009
• Publication Date: Sep 15, 2009
• Deposit Date: Sep 21, 2016
• Journal: Journal of Cell Science
• Print ISSN: 0021-9533
• Electronic ISSN: 1477-9137
• Publisher: Company of Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 122
• Issue: 18
• Pages: 3414-3423
• DOI: https://doi.org/10.1242/jcs.044610
• Keywords: Invadolysin, Lipid droplets, Metalloprotease, Phylogeny
• Public URL: http://researchrepository.napier.ac.uk/Output/369727