Skip to main content

Research Repository

Advanced Search

A holidic medium for Drosophila melanogaster

Piper, Matthew D W; Blanc, Eric; Leitão-Gonçalves, Ricardo; Yang, Mingyao; He, Xiaoli; Linford, Nancy J; Hoddinott, Matthew P; Hopfen, Corinna; Soultoukis, George A; Niemeyer, Christine; Kerr, Fiona; Pletcher, Scott D; Ribeiro, Carlos; Partridge, Linda

Authors

Matthew D W Piper

Eric Blanc

Ricardo Leitão-Gonçalves

Mingyao Yang

Xiaoli He

Nancy J Linford

Matthew P Hoddinott

Corinna Hopfen

George A Soultoukis

Christine Niemeyer

Scott D Pletcher

Carlos Ribeiro

Linda Partridge



Abstract

A critical requirement for research using model organisms is a well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. This holidic diet supports development over multiple generations but at a reduced rate. Over 7 years of experiments, the holidic diet yielded more consistent experimental outcomes than did oligidic food for egg laying by females. Nutrients and drugs were more available to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increased fly lifespan. We used this holidic medium to investigate amino acid–specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development.

Citation

Piper, M. D. W., Blanc, E., Leitão-Gonçalves, R., Yang, M., He, X., Linford, N. J., Hoddinott, M. P., Hopfen, C., Soultoukis, G. A., Niemeyer, C., Kerr, F., Pletcher, S. D., Ribeiro, C., & Partridge, L. (2014). A holidic medium for Drosophila melanogaster. Nature methods, 11(1), 100-105. https://doi.org/10.1038/nmeth.2731

Journal Article Type Article
Acceptance Date Oct 10, 2013
Online Publication Date Nov 17, 2013
Publication Date 2014-01
Deposit Date Aug 19, 2016
Journal Nature Methods
Electronic ISSN 1548-7091
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 11
Issue 1
Pages 100-105
DOI https://doi.org/10.1038/nmeth.2731
Keywords Behavioural genetics, Drosophila, Feeding behaviour, Metabolism
Public URL http://researchrepository.napier.ac.uk/Output/355716



You might also like



Downloadable Citations