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A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae

Simmons, P.; Singleton, I.

Authors

P. Simmons



Abstract

Ag+ biosorption by an industrial strain of Saccharomyces cerevisiae was investigated. Older (96 h old) biomass had half the biosorption capacity of younger (24 h old) biomass (0.187 and 0.387 mmol Ag+/g dry mass respectively). Comparisons of cell walls isolated from biomass of either age indicated that chemical composition and Ag+ biosorption capacity varied little over the time span examined and that cell walls from either age of culture had small Ag+ biosorption capacities compared to whole cells of a similar age. Silver-containing precipitates were observed both on the cell wall and within the cell, indicating that intracellular components sorbed Ag+. The concentration of these precipitates within the cell appeared visually to decrease with age in Ag+-exposed cells. Incorporation of l-cysteine into the growth medium resulted in biomass with increased silver biosorption capacities, protein and sulphydryl group content. Increasing the concentration of l-cysteine in the growth medium from 0 to 5.0 mM increased silver biosorption from 0.389 to 0.556 mmol Ag+/g dry mass Isolated cell walls of biomass grown in supplemented media also showed a possible link between silver biosorption capacities, protein and sulphydryl group content. No precipitates were observed in silver-exposed biomass that had been grown in the presence of 5.0 mM l-cysteine.

Citation

Simmons, P., & Singleton, I. (1996). A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae. Applied microbiology and biotechnology, 45(1-2), 278-285. https://doi.org/10.1007/s002530050684

Journal Article Type Article
Acceptance Date Aug 22, 1995
Publication Date 1996-03
Deposit Date Aug 5, 2016
Journal Appl Microbiol Biotechnol; Applied Microbiology and Biotechnology
Print ISSN 1432-0614
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 45
Issue 1-2
Pages 278-285
DOI https://doi.org/10.1007/s002530050684
Keywords Biomass, Cell Wall, Biosorption, Biosorption Capacity, Cell Wall Composition ,
Public URL http://researchrepository.napier.ac.uk/Output/328249