P. Simmons
A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae
Simmons, P.; Singleton, I.
Abstract
Ag+ biosorption by an industrial strain of Saccharomyces cerevisiae was investigated. Older (96 h old) biomass had half the biosorption capacity of younger (24 h old) biomass (0.187 and 0.387 mmol Ag+/g dry mass respectively). Comparisons of cell walls isolated from biomass of either age indicated that chemical composition and Ag+ biosorption capacity varied little over the time span examined and that cell walls from either age of culture had small Ag+ biosorption capacities compared to whole cells of a similar age. Silver-containing precipitates were observed both on the cell wall and within the cell, indicating that intracellular components sorbed Ag+. The concentration of these precipitates within the cell appeared visually to decrease with age in Ag+-exposed cells. Incorporation of l-cysteine into the growth medium resulted in biomass with increased silver biosorption capacities, protein and sulphydryl group content. Increasing the concentration of l-cysteine in the growth medium from 0 to 5.0 mM increased silver biosorption from 0.389 to 0.556 mmol Ag+/g dry mass Isolated cell walls of biomass grown in supplemented media also showed a possible link between silver biosorption capacities, protein and sulphydryl group content. No precipitates were observed in silver-exposed biomass that had been grown in the presence of 5.0 mM l-cysteine.
Citation
Simmons, P., & Singleton, I. (1996). A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae. Applied microbiology and biotechnology, 45(1-2), 278-285. https://doi.org/10.1007/s002530050684
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 22, 1995 |
Publication Date | 1996-03 |
Deposit Date | Aug 5, 2016 |
Journal | Appl Microbiol Biotechnol; Applied Microbiology and Biotechnology |
Print ISSN | 1432-0614 |
Publisher | Springer |
Peer Reviewed | Peer Reviewed |
Volume | 45 |
Issue | 1-2 |
Pages | 278-285 |
DOI | https://doi.org/10.1007/s002530050684 |
Keywords | Biomass, Cell Wall, Biosorption, Biosorption Capacity, Cell Wall Composition , |
Public URL | http://researchrepository.napier.ac.uk/Output/328249 |
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