Jean-Pascal Piret
Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles
Piret, Jean-Pascal; Bondarenko, Olesja M.; Boyles, Matthew S. P.; Himly, Martin; Ribeiro, Ana R.; Benetti, Federico; Smal, Caroline; Lima, Braulio; Potthoff, Annegret; Simion, Monica; Dumortier, Elise; Leite, Paulo Emilio C.; Balottin, Luciene Bottentuit; Granjeiro, José Mauro; Ivask, Angela; Kahru, Anne; Radauer-Preiml, Isabella; Tischler, Ulrike; Duschl, Albert; Saout, Christelle; Anguissola, Sergio; Haase, Andrea; Jacobs, An; Nelissen, Inge; Misra, Superb K.; Toussaint, Olivier
Authors
Olesja M. Bondarenko
Dr Matthew Boyles M.Boyles2@napier.ac.uk
Lecturer
Martin Himly
Ana R. Ribeiro
Federico Benetti
Caroline Smal
Braulio Lima
Annegret Potthoff
Monica Simion
Elise Dumortier
Paulo Emilio C. Leite
Luciene Bottentuit Balottin
José Mauro Granjeiro
Angela Ivask
Anne Kahru
Isabella Radauer-Preiml
Ulrike Tischler
Albert Duschl
Christelle Saout
Sergio Anguissola
Andrea Haase
An Jacobs
Inge Nelissen
Superb K. Misra
Olivier Toussaint
Abstract
The rapid development of nanotechnologies and increased production and use of nanomaterials raise concerns about their potential toxic effects for human health and environment. To evaluate the biological effects of nanomaterials, a set of reliable and reproducible methods and development of standard operating procedures (SOPs) is required. In the framework of the European FP7 NanoValid project, three different cell viability assays (MTS, ATP content, and caspase-3/7 activity) with different readouts (absorbance, luminescence and fluorescence) and two immune assays (ELISA of pro-inflammatory cytokines IL1-β and TNF-α) were evaluated by inter-laboratory comparison. The aim was to determine the suitability and reliability of these assays for nanosafety assessment. Studies on silver and copper oxide nanoparticles (NPs) were performed, and SOPs for particle handling, cell culture, and in vitro assays were established or adapted. These SOPs give precise descriptions of assay procedures, cell culture/seeding conditions, NPs/positive control preparation and dilutions, experimental well plate preparation, and evaluation of NPs interference. The following conclusions can be highlighted from the pan-European inter-laboratory studies: Testing of NPs interference with the toxicity assays should always be conducted. Interference tests should be designed as close as possible to the cell exposure conditions. ATP and MTS assays gave consistent toxicity results with low inter-laboratory variability using Ag and CuO NPs and different cell lines and therefore, could be recommended for further validation and standardization. High inter-laboratory variability was observed for Caspase 3/7 assay and ELISA for IL1-β and TNF-α measurements.
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 24, 2016 |
Online Publication Date | Dec 10, 2016 |
Publication Date | 2017-06 |
Deposit Date | Oct 13, 2023 |
Print ISSN | 0340-5761 |
Electronic ISSN | 1432-0738 |
Publisher | Springer |
Peer Reviewed | Peer Reviewed |
Volume | 91 |
Issue | 6 |
Pages | 2315-2330 |
DOI | https://doi.org/10.1007/s00204-016-1897-2 |
Keywords | Inter-laboratory studies, Standard operating procedures, Interference, In vitro assays, Nanoparticles |
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