Dr Clare Taylor Cl.Taylor@napier.ac.uk
Senior Lecturer
Mutations in the waaR Gene of Escherichia coli Which Disrupt Lipopolysaccharide Outer Core Biosynthesis Affect Cell Surface Retention of Group 2 Capsular Polysaccharides.
Taylor, Clare M.; Goldrick, Marie; Lord, Liz; Roberts, Ian S.
Authors
Marie Goldrick
Liz Lord
Ian S. Roberts
Abstract
On the basis of increased resistance to K5 capsule-specific bacteriophage, a waaR transposon mutant defective in the biosynthesis of lipopolysaccharide outer core was isolated. In a K1-expressing strain the mutation equally affected sensitivity to K1 capsule-specific bacteriophage, indicating a general effect on group 2 capsules. The waaR mutation affected retention on the cell surface of the K5 polysaccharide, with increased polysaccharide accumulating in the culture supernatant. This indicates that interactions between the outer core of lipopolysaccharide and group 2 capsular polysaccharides are important for the stabilization of group 2 capsular polysaccharides on the cell surface.
Citation
Taylor, C. M., Goldrick, M., Lord, L., & Roberts, I. S. (2006). Mutations in the waaR Gene of Escherichia coli Which Disrupt Lipopolysaccharide Outer Core Biosynthesis Affect Cell Surface Retention of Group 2 Capsular Polysaccharides. Journal of Bacteriology, 188(3), 1165-1168. https://doi.org/10.1128/JB.188.3.1165-1168.2006
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 31, 2005 |
Publication Date | 2006 |
Deposit Date | Jul 27, 2016 |
Journal | Journal of Bacteriology |
Print ISSN | 0021-9193 |
Electronic ISSN | 1098-5530 |
Publisher | American Society for Microbiology |
Peer Reviewed | Peer Reviewed |
Volume | 188 |
Issue | 3 |
Pages | 1165-1168 |
DOI | https://doi.org/10.1128/JB.188.3.1165-1168.2006 |
Keywords | Mutations, waaR gene, |
Public URL | http://researchrepository.napier.ac.uk/Output/318097 |
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