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LC/MS-based discrimination between plasma and urine metabolomic changes following exposure to ultraviolet radiation by using data modelling

Ali, Ali Muhsen; Monaghan, Chris; Muggeridge, David J.; Easton, Chris; Watson, David G.

Authors

Ali Muhsen Ali

Chris Monaghan

Chris Easton

David G. Watson



Abstract

Introduction: This study sought to compare between metabolomic changes of human urine and plasma to investigate which one can be used as best tool to identify metabolomic profiling and novel biomarkers associated to the potential effects of ultraviolet (UV) radiation. Method: A pilot study of metabolomic patterns of human plasma and urine samples from four adult healthy individuals at before (S1) and after (S2) exposure (UV) and non-exposure (UC) were carried out by using liquid chromatography-mass spectrometry (LC–MS). Results: The best results which were obtained by normalizing the metabolites to their mean output underwent to principal components analysis (PCA) and Orthogonal Partial least squares-discriminant analysis (OPLS-DA) to separate pre-from post-of exposure and non-exposure of UV. This separation by data modeling was clear in urine samples unlike plasma samples. In addition to overview of the scores plots, the variance predicted-Q2 (Cum), variance explained-R2X (Cum) and p-value of the cross-validated ANOVA score of PCA and OPLS-DA models indicated to this clear separation. Q2 (Cum) and R2X (Cum) values of PCA model for urine samples were 0.908 and 0.982, respectively, and OPLS-DA model values were 1.0 and 0.914, respectively. While these values in plasma samples were Q2 = 0.429 and R2X = 0.660 for PCA model and Q2 = 0.983 and R2X = 0.944 for OPLS-DA model. LC–MS metabolomic analysis showed the changes in numerous metabolic pathways including: amino acid, lipids, peptides, xenobiotics biodegradation, carbohydrates, nucleotides, Co-factors and vitamins which may contribute to the evaluation of the effects associated with UV sunlight exposure. Conclusions: The results of pilot study indicate that pre and post-exposure UV metabolomics screening of urine samples may be the best tool than plasma samples and a potential approach to predict the metabolomic changes due to UV exposure. Additional future work may shed light on the application of available metabolomic approaches to explore potential predictive markers to determine the impacts of UV sunlight.

Journal Article Type Article
Acceptance Date Jan 23, 2023
Online Publication Date Feb 13, 2023
Publication Date 2023
Deposit Date Feb 20, 2023
Publicly Available Date Feb 20, 2023
Journal Metabolomics
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 19
Issue 2
Article Number 13
DOI https://doi.org/10.1007/s11306-023-01977-0
Keywords LC–MS, Ultraviolet radiation (UV), Urine, Plasma, Metabolomic profiling, Principal components analysis (PCA), Orthogonal partial least squares- discriminant analysis (OPLS-DA)

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http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.





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