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Structural optimization of imidazothiazole derivatives affords a new promising series as B-Raf V600E inhibitors; synthesis, in vitro assay and in silico screening

Ammar, Usama M.; Abdel-Maksoud, Mohammed S.; Ali, Eslam M.H.; Mersal, Karim I.; Ho Yoo, Kyung; Oh, Chang-Hyun


Mohammed S. Abdel-Maksoud

Eslam M.H. Ali

Karim I. Mersal

Kyung Ho Yoo

Chang-Hyun Oh


BRAF mutation is commonly known in a number of human cancer types. It is counted as a potential component in treating cancer. In this study, based on structural optimization of previously reported inhibitors (3-fluro substituted derivatives of imidazo[2,1-b]thiazole-based scaffold), we designed and synthesized sixteen new imidazo[2,1-b]thiazole derivatives with m-nitrophenyl group at position 6. The electron withdrawing properties was reserved while the polarity was modified compared to previously synthesized compounds (-F). Furthermore, the new substituted group (-NO 2) provided an additional H-bond acceptor(s) which may bind with the target enzyme through additional interaction(s). In vitro cytotoxicity evaluation was performed against human cancer cell line (A375). In addition, in vitro enzyme assay was performed against mutated B-Raf (B-Raf V600E). Compounds 13a, 13g and 13f showed highest activity on mutated B-Raf with IC 50 0.021, 0.035 and 0.020 µM. All target compounds were tested for in vitro cytotoxicity against NCI 60 cell lines. Compounds 13a and 13g were selected for 5 doses test mode. Moreover, in silico molecular simulation was explored in order to explore the possible interactions between the designed compounds and the B-Raf V600E active site.

Journal Article Type Article
Acceptance Date May 20, 2020
Online Publication Date May 22, 2020
Publication Date 2020-07
Deposit Date Dec 11, 2022
Journal Bioorganic Chemistry
Print ISSN 0045-2068
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 100
Article Number 103967
Keywords Anti-cancer, B-Raf V600E, Cancer, Imidazo[2,1-b]thiazole, In silico screening
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