Jonas S. G. Silvander
Keratins regulate β‐cell mitochondrial morphology, motility, and homeostasis
Silvander, Jonas S. G.; Kvarnström, Sofie M.; Kumari‐Ilieva, Angeli; Shrestha, Anup; Alam, Catharina M.; Toivola, Diana M.
Authors
Sofie M. Kvarnström
Angeli Kumari‐Ilieva
Anup Shrestha
Dr Catharina Alam C.Alam@napier.ac.uk
Lecturer
Diana M. Toivola
Abstract
Loss of the epithelial intermediate filament protein keratin 8 (K8) in murine β cells leads to irregular insulin vesicles and decreased insulin levels. Because mitochondria are central in glucose-stimulated insulin secretion, the relationship between keratins and β-cell mitochondrial function and morphology was investigated. β cells in murine K8-knockout (K8−/−) islets of Langerhans have increased numbers of mitochondria, which are rounder and have diffuse cristae, as seen by electron microscopy. The mitochondrial network in primary cultured K8−/− β cells is more fragmented compared with K8+/+ mitochondria, correlating with decreased levels of mitofusin 2 and the mitofusin 2- and keratin-binding protein trichoplein. K8−/− β-cell mitochondria have decreased levels of total and mitochondrial cytochrome c, which correlates with a reduction in electron transport complexes I and IV. This provokes loss of mitochondrial membrane potential and reduction of ATP and insulin amount, as seen in K8−/− β cells. Mitochondria in K8 wild-type β cells and MIN6 insulinoma cells overexpressing K8 and 18 are more stationary compared with mitochondria in keratin-deficient cells. In conclusion, keratins, likely through trichoplein-mitofusin interactions, regulate both structural and dynamic functions of β-cell mitochondria, which could have implications for downstream insulin secretion.
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 19, 2017 |
Online Publication Date | Sep 19, 2017 |
Publication Date | 2017-10 |
Deposit Date | Nov 14, 2022 |
Journal | The FASEB Journal |
Print ISSN | 0892-6638 |
Electronic ISSN | 1530-6860 |
Publisher | Federation of American Society of Experimental Biology |
Peer Reviewed | Peer Reviewed |
Volume | 31 |
Issue | 10 |
Pages | 4578-4587 |
DOI | https://doi.org/10.1096/fj.201700095r |
Keywords | TEM, insulin secretion, islets, K8-null mice, fusion |
Public URL | http://researchrepository.napier.ac.uk/Output/2957226 |
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