Incompatibility properties of the narrow-host-range lactococcal plasmid pCI305.

Foley, Sophie; Daly, Charles; Fitzgerald, Gerald F

Abstract

The cryptic plasmid pCI305 from Lactococcus Zuctis subsp. lactis UC317 was screened for incompatibility with
a range of cloning vectors and was found to be incompatible with the theta-replicating plasmids pAM401 and
pIL253. In contrast, lactococcal vectors containing the replication functions of the rolling circle replicating
plasmids pSH71 and pWVOl were compatible with pCI305. A number of native lactococcal plasmids were also
screened for both incompatibility and DNA sequence homology to the pCI305 replicon. A plasmid with high
sequence identity was identified which was compatible with pCI305.

Citation

Foley, S., Daly, C., & Fitzgerald, G. F. (1993). Incompatibility properties of the narrow-host-range lactococcal plasmid pCI305. The Journal of general microbiology, 139, 1271-1276. https://doi.org/10.1099/00221287-139-6-1271

• Journal Article Type: Article
• Acceptance Date: Jan 29, 1993
• Publication Date: 1993-06
• Deposit Date: May 3, 2016
• Publicly Available Date: May 3, 2016
• Print ISSN: 0022-1287
• Peer Reviewed: Peer Reviewed
• Volume: 139
• Pages: 1271-1276
• DOI: https://doi.org/10.1099/00221287-139-6-1271
• Keywords: cryptic plasmid pCI305; Lactococcus lactis; lactis UC317; theta-replicating plasmids pAM401; cloning vectors
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/9935
• Publisher URL: http://dx.doi.org/10.1099/00221287-139-6-1271
• Contract Date: May 3, 2016