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Expression of transforming growth factor beta mRNA isoforms in human breast cancer

MacCallum, J.; Bartlett, J.M.S.; Thompson, A.M.; Keen, J.C.; Dixon, J.M.; Miller, W.R.


J.M.S. Bartlett

A.M. Thompson

J.C. Keen

J.M. Dixon

W.R. Miller


Using an RNAse protection assay, expression of messenger RNA for isoforms of TGF-beta was determined in a series of breast cancers. Of 50 tumours, 45 (90%) expressed TGF-beta 1 mRNA, 39 (78%) expressed TGF-beta 2, and 47 (94%) expressed TGF-beta 3. Patterns of expression varied between different tumours: 37 (74%) cancers expressed all three TGF-beta isoforms, ten (20%) expressed only two isoforms and two expressed TGF-beta 1 alone. One sample showed no evidence of TGF-beta mRNA expression. Although most breast cancers expressed mRNA for at least one isoform of TGF-beta, there were differences in patterns of mRNA expression between individual tumours. The relatively small number of tumours examined precluded detailed analysis between expression and other clinical parameters, but a significant association was identified between one aspect of isoform expression and lymph node status, in that the majority of tumours expressing all three isoforms were associated with lymph node involvement, whereas tumours without one or more isoform were usually lymph node negative (P = 0.025 by Fisher's exact test).

Journal Article Type Article
Publication Date 1994-06
Deposit Date May 30, 2022
Journal British Journal of Cancer
Print ISSN 0007-0920
Electronic ISSN 1532-1827
Publisher Cancer Research UK
Peer Reviewed Peer Reviewed
Volume 69
Issue 6
Pages 1006-1009
Keywords Cancer Research; Oncology
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