DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains.

Morrison, Donald; Woodford, N; Barrett, S P; Sisson, P; Cookson, B D

DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains.

Morrison, Donald; Woodford, N; Barrett, S P; Sisson, P; Cookson, B D

Authors

Dr Donald Morrison D.Morrison2@napier.ac.uk
Associate Professor

N Woodford

S P Barrett

P Sisson

B D Cookson

Abstract

The degree of DNA banding pattern polymorphism exhibited by vancomycin-resistant Enterococcus faecium (VREM) strains isolated on a renal unit over an 11-month period was investigated. Thirty VREM strains from different patients were analyzed by pulsed-field gel electrophoresis (PFGE; with extended run and optimal pulse times), ribotyping, plasmid profile analysis, biotyping, pyrolysis mass spectrometry, and antibiogram analysis. PFGE resolved 17 banding patterns which formed four distinct clusters at the 82% similarity level. Intercluster band differences ranged from 14 to 31 bands. The strains in one cluster, which contained seven patterns that differed from each other by one to seven bands and from the common pattern by five bands, were confirmed to be a single strain by four of the five other typing methods. The strains in a second cluster with eight patterns, which differed from each other by 1 to 12 bands, contained two subclusters. This subdivision was supported by ribotyping and biotyping. However, it was unclear whether these subclusters represented distinct strains. In one strain, marked polymorphism (patterns that differed from each other by up to four bands) was observed in the ribotype pattern. This study demonstrates the high degree of DNA banding pattern polymorphism found for some strains of VREM and illustrates the complexity involved in defining such strains.

Citation

Morrison, D., Woodford, N., Barrett, S. P., Sisson, P., & Cookson, B. D. (1999). DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains. Journal of Clinical Microbiology, 37, 1084-1091

Journal Article Type	Article
Publication Date	1999
Deposit Date	Jun 11, 2015
Print ISSN	0095-1137
Electronic ISSN	1098-660X
Publisher	American Society for Microbiology
Peer Reviewed	Peer Reviewed
Volume	37
Pages	1084-1091
Keywords	DNA banding pattern polymorphism; vancomycin-resistant Enterococcus faecium (VREM); pulsed-field gel electrophoresis (PFGE);
Public URL	http://researchrepository.napier.ac.uk/id/eprint/8659