Single-sweep voltage-sensitive dye imaging of interacting identified neurons

Stein, Wolfgang; St�dele, Carola; Andras, Peter

Abstract

The simultaneous recording of many individual neurons is fundamental to understanding the integral functionality of neural systems. Imaging with voltage-sensitive dyes (VSDs) is a key approach to achieve this goal and a promising technique to supplement electrophysiological recordings. However, the lack of connectivity maps between imaged neurons and the requirement of averaging over repeated trials impede functional interpretations. Here we demonstrate fast, high resolution and single-sweep VSD imaging of identified and synaptically interacting neurons. We show for the first time the optical recording of individual action potentials and mutual inhibitory synaptic input of two key players in the well-characterized pyloric central pattern generator in the crab stomatogastric ganglion (STG). We also demonstrate the presence of individual synaptic potentials from other identified circuit neurons. We argue that imaging of neural networks with identifiable neurons with well-known connectivity, like in the STG, is crucial for the understanding of emergence of network functionality.

• Journal Article Type: Article
• Acceptance Date: Oct 8, 2010
• Online Publication Date: Oct 20, 2010
• Publication Date: 2011-01
• Deposit Date: Nov 2, 2021
• Publicly Available Date: Nov 2, 2021
• Journal: Journal of neuroscience methods
• Print ISSN: 0165-0270
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 194
• Issue: 2
• Pages: 224-234
• DOI: https://doi.org/10.1016/j.jneumeth.2010.10.007
• Keywords: Voltage-sensitive dye, Stomatogastric ganglion, Neuron resolution imaging, Neuron interaction imaging
• Public URL: http://researchrepository.napier.ac.uk/Output/2808785