Katherine A. Staines
Proton Pump Inhibitors Inhibit PHOSPHO1 Activity and Matrix Mineralisation In Vitro
Staines, Katherine A.; Myers, Katherine; Little, Kirsty; Ralston, Stuart H.; Farquharson, Colin
Authors
Katherine Myers
Kirsty Little
Stuart H. Ralston
Colin Farquharson
Abstract
Proton pump inhibitors (PPIs) have been associated with an increased risk of fragility fractures in pharmaco-epidemiological studies. The mechanism is unclear, but it has been speculated that by neutralising gastric acid, they may reduce intestinal calcium absorption, causing secondary hyperparathyroidism and bone loss. Here we investigated that hypothesis that the skeletal effects of PPI might be mediated by inhibitory effects on the bone-specific phosphatase PHOSPHO1. We found that the all PPIs tested inhibited the activity of PHOSPHO1 with IC50 ranging between 0.73 µM for esomeprazole to 19.27 µM for pantoprazole. In contrast, these PPIs did not inhibit TNAP activity. We also found that mineralisation of bone matrix in primary osteoblast cultures was inhibited by several PPIs in a concentration dependent manner. In contrast, the histamine-2 receptor antagonists (H2RA) nizatidine, famotidine, cimetidine and ranitidine had no inhibitory effects on PHOSPHO1 activity. Our experiments show for the first time that PPIs inhibit PHOSPHO1 activity and matrix mineralisation in vitro revealing a potential mechanism by which these widely used drugs are associated with the risk of fractures.
Citation
Staines, K. A., Myers, K., Little, K., Ralston, S. H., & Farquharson, C. (2021). Proton Pump Inhibitors Inhibit PHOSPHO1 Activity and Matrix Mineralisation In Vitro. Calcified Tissue International, 109, 696-705. https://doi.org/10.1007/s00223-021-00882-9
Journal Article Type | Article |
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Acceptance Date | Jun 19, 2021 |
Online Publication Date | Jul 2, 2021 |
Publication Date | 2021-12 |
Deposit Date | Jul 12, 2021 |
Publicly Available Date | Jul 12, 2021 |
Journal | Calcified Tissue International |
Print ISSN | 0171-967X |
Electronic ISSN | 1432-0827 |
Publisher | Springer |
Peer Reviewed | Peer Reviewed |
Volume | 109 |
Pages | 696-705 |
DOI | https://doi.org/10.1007/s00223-021-00882-9 |
Keywords | PHOSPHO1, Proton pump inhibitors, Histamine-2 receptor antagonists, Mineralisation, TNAP |
Public URL | http://researchrepository.napier.ac.uk/Output/2786163 |
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Proton Pump Inhibitors Inhibit PHOSPHO1 Activity And Matrix Mineralisation In Vitro
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