Conserved queen pheromones in bumblebees: a reply to Amsalem et al.

Holman, Luke; van Zweden, Jelle S.; Oliveira, Ricardo C.; van Oystaeyen, Annette; Wenseleers, Tom

Abstract

In a recent study, Amsalem, Orlova & Grozinger (2015) performed experiments with Bombus impatiens bumblebees to test the hypothesis that saturated cuticular hydrocarbons are evolutionarily conserved signals used to regulate reproductive division of labor in many Hymenopteran social insects. They concluded that the cuticular hydrocarbon pentacosane (C25), previously identified as a queen pheromone in a congeneric bumblebee, does not affect worker reproduction in B. impatiens. Here we discuss some shortcomings of Amsalem et al.’s study that make its conclusions unreliable. In particular, several confounding effects may have affected the results of both experimental manipulations in the study. Additionally, the study’s low sample sizes (mean n per treatment = 13.6, range: 4–23) give it low power, not 96–99% power as claimed, such that its conclusions may be false negatives. Inappropriate statistical tests were also used, and our reanalysis found that C25 substantially reduced and delayed worker egg laying in B. impatiens. We review the evidence that cuticular hydrocarbons act as queen pheromones, and offer some recommendations for future queen pheromone experiments.

Citation

Holman, L., van Zweden, J. S., Oliveira, R. C., van Oystaeyen, A., & Wenseleers, T. (2017). Conserved queen pheromones in bumblebees: a reply to Amsalem et al. PeerJ, 5, Article e3332. https://doi.org/10.7717/peerj.3332

• Journal Article Type: Article
• Acceptance Date: Apr 16, 2017
• Online Publication Date: May 16, 2017
• Publication Date: May 16, 2017
• Deposit Date: Apr 14, 2021
• Publicly Available Date: Apr 15, 2021
• Journal: PeerJ
• Electronic ISSN: 2167-8359
• Publisher: PeerJ
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Article Number: e3332
• DOI: https://doi.org/10.7717/peerj.3332
• Keywords: Eusociality, Cuticular hydrocarbons, Fertility signals, Reproductive division of labour
• Public URL: http://researchrepository.napier.ac.uk/Output/2761512