Prolonged activity of a recombinant manganese superoxide dismutase through a formulation of polymeric multi-layer nanoassemblies targeting cancer cells

Russo, Giacomo; Iaccarino, Giulia; Piccolo, Marialuisa; Ferraro, Maria Grazia; Vecchione, Raffaele; Grumetto, Lucia; Netti, Paolo A.; Santamaria, Rita

Abstract

A new isoform of human manganese superoxide dismutase (SOD) has been recently isolated and obtained in a synthetic recombinant form and termed rMnSOD. As compared to other SODs, this isoform exhibits a dramatically improved cellular uptake and an intense antioxidant and antitumoral activity. Unfortunately, its use is severely hampered as this active pharmaceutical ingredient (API) in solution suffers from remarkable instability, which realizes as an interplay of unfolding and aggregation phenomena. This leads the API to be ineffective after three weeks only when stored at 4°C.

Citation

Russo, G., Iaccarino, G., Piccolo, M., Ferraro, M. G., Vecchione, R., Grumetto, L., Netti, P. A., & Santamaria, R. (2021). Prolonged activity of a recombinant manganese superoxide dismutase through a formulation of polymeric multi-layer nanoassemblies targeting cancer cells. European Journal of Pharmaceutical Sciences, 162, Article 105825. https://doi.org/10.1016/j.ejps.2021.105825

• Journal Article Type: Article
• Acceptance Date: Mar 25, 2021
• Online Publication Date: Apr 2, 2021
• Publication Date: 2021-07
• Deposit Date: Apr 2, 2021
• Publicly Available Date: Apr 3, 2022
• Journal: European Journal of Pharmaceutical Sciences
• Print ISSN: 0928-0987
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 162
• Article Number: 105825
• DOI: https://doi.org/10.1016/j.ejps.2021.105825
• Keywords: Superoxide dismutase, cancer cells, antiproliferative effect, protein stability, nanocarrier, layer-by-layer deposition, nanofabrication
• Public URL: http://researchrepository.napier.ac.uk/Output/2758700