Skip to main content

Research Repository

Advanced Search

Development and Validation of a LC-FD Method for the Simultaneous Determination of Eight Bisphenols in Soft Drinks

Russo, Giacomo; Barbato, Francesco; Grumetto, Lucia


Francesco Barbato

Lucia Grumetto


Bisphenol A (BPA) is a chemical widely used as a monomer in the production of polymers of plastics. It acts as an endocrine-disrupting agent and thus its contaminations of food and beverage should be carefully monitored in order to assess consumers’ risk. In this study, we propose a liquid chromatography-fluorescence detection (LC-FD)-validated method for the simultaneous determination of BPA and seven analogues, i.e., bisphenol AF, bisphenol B, bisphenol E, bisphenol F, BPA diglycidyl ether, bisphenol F diglycidyl ether, and Bisphenol M in soft drinks. A one-step solid-phase extraction (SPE) was effective at reducing the interferences, obtaining good purification of the samples, and consequently good recoveries of all analytes. The separation was obtained on a C18 column by using acetonitrile/water 55:45 (v/v) under isocratic conditions. Method validation was performed according to the European Commission Decision 2002/657/EC criteria, providing good results regarding the analytical parameters of linearity, selectivity, sensitivity, precision, recovery, decision limit (CCα), detection capability (CCβ), limit of detection (LOD), limit of quantification (LOQ), stability, and robustness. The method allows the detection of BPA and BADGE at levels much lower than their legal limits in the food, which are 0.06 and 9.00 mg kg−1, respectively.


Russo, G., Barbato, F., & Grumetto, L. (2016). Development and Validation of a LC-FD Method for the Simultaneous Determination of Eight Bisphenols in Soft Drinks. Food Analytical Methods, 9(10), 2732-2740.

Journal Article Type Article
Acceptance Date Feb 22, 2016
Online Publication Date Mar 3, 2016
Publication Date 2016-10
Deposit Date May 4, 2020
Journal Food Analytical Methods
Print ISSN 1936-9751
Electronic ISSN 1936-976X
Publisher BMC
Peer Reviewed Peer Reviewed
Volume 9
Issue 10
Pages 2732-2740
Keywords Food Science; Analytical Chemistry; Applied Microbiology and Biotechnology; Safety Research; Safety, Risk, Reliability and Quality
Public URL