Dr Giacomo Russo G.Russo@napier.ac.uk
Lecturer
Cytotoxicity of seven bisphenol analogues compared to bisphenol A and relationships with membrane affinity data
Russo, Giacomo; Capuozzo, Antonella; Barbato, Francesco; Irace, Carlo; Santamaria, Rita; Grumetto, Lucia
Authors
Antonella Capuozzo
Francesco Barbato
Carlo Irace
Rita Santamaria
Lucia Grumetto
Abstract
Bisphenol A (BPA) is a chemical used in numerous industrial applications. Due to its well ascertained toxicity as endocrine disruptor, industries have started to replace it with other bisphenols whose alleged greater safety is scarcely supported by literature studies. In this study, the toxicity of seven BPA analogues was evaluated using both in silico and in vitro techniques, as compared to BPA toxicity. Furthermore, their affinity indexes for phospholipids (i.e. phospholipophilicity) were determined by immobilized artificial membrane liquid chromatography (IAM-LC) and possible relationships with in vitro toxic activity were also investigated. The results on four different cell cultures yielded similar ranking of toxicity for the bisphenols considered, with IC50 values confirming their poor acute toxicity. As compared to BPA, bisphenol AF, bisphenol B, bisphenol M, and bisphenol A diglycidyl ether resulted more toxic, while bisphenol S, bisphenol F and bisphenol E were found as the less toxic congeners. These results are partly consistent with the scale of phospholipid affinity showing that toxicity increases at increasing membrane affinity. Therefore, phospholipophilicity determination can be assumed as a useful preliminary tool to select less toxic congeners to surrogate BPA in industrial applications.
Citation
Russo, G., Capuozzo, A., Barbato, F., Irace, C., Santamaria, R., & Grumetto, L. (2018). Cytotoxicity of seven bisphenol analogues compared to bisphenol A and relationships with membrane affinity data. Chemosphere, 201, 432-440. https://doi.org/10.1016/j.chemosphere.2018.03.014
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Mar 3, 2018 |
| Online Publication Date | Mar 5, 2018 |
| Publication Date | 2018-06 |
| Deposit Date | May 4, 2020 |
| Journal | Chemosphere |
| Print ISSN | 0045-6535 |
| Electronic ISSN | 1879-1298 |
| Publisher | Elsevier |
| Peer Reviewed | Peer Reviewed |
| Volume | 201 |
| Pages | 432-440 |
| DOI | https://doi.org/10.1016/j.chemosphere.2018.03.014 |
| Keywords | Bisphenol, Immobilized artificial membrane, ADMET predictor, Cell viability, Toxicity, Endocrine disrupting chemicals |
| Public URL | http://researchrepository.napier.ac.uk/Output/2658176 |