Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections.

Shittu, Adebayo; Lin, Johnson; Morrison, Donald; Kolawole, Deboye

Abstract

The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to identify an isolate of S. haemolyticus fully. The PCR assay, which detects polymorphism in the 16S–23S rRNA spacer region, is shown to be potentially useful for rapid and accurate identification of coagulase-negative staphylococci. Identical PFGE type and antibiotic-resistance profiles of two methicillin-resistant S. haemolyticus isolates in this study suggest the existence of a multiresistant community clone.

Citation

Shittu, A., Lin, J., Morrison, D., & Kolawole, D. (2004). Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections. Journal of Medical Microbiology, 53, 51-55. https://doi.org/10.1099/jmm.0.05294-0

• Journal Article Type: Article
• Publication Date: 2004
• Deposit Date: Jun 12, 2015
• Print ISSN: 0022-2615
• Electronic ISSN: 1473-5644
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 53
• Pages: 51-55
• DOI: https://doi.org/10.1099/jmm.0.05294-0
• Keywords: Staphylococcus sciuri; Staphylococcus haemolyticus; multiresistance; skin and soft-tissue infections
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/8671
• Publisher URL: http://dx.doi.org/10.1099/jmm.0.05294-0