A complete shikimate pathway in Toxoplasma gondii: an ancient eukaryotic innovation

Campbell, S.A.; Campbell, Samantha A; Richards, T.A.; Mui, E.J.; Samuel, B.U.; Coggins, J.R.; McLeod, R.; Roberts, C.W.

Abstract

The shikimate pathway is essential for survival of the apicomplexan parasites Plasmodium falciparum, Toxoplasma gondii and Cryptosporidium parvum. As it is absent in mammals it is a promising therapeutic target. Herein, we describe the genes encoding the shikimate pathway enzymes in T. gondii. The molecular arrangement and phylogeny of the proteins suggests homology with the eukaryotic fungal enzymes, including a pentafunctional AROM. Current rooting of the eukaryotic evolutionary tree infers that the fungi and apicomplexan lineages diverged deeply, suggesting that the arom is an ancient supergene present in early eukaryotes and subsequently lost or replaced in a number of lineages.

Citation

Campbell, S., Campbell, S. A., Richards, T., Mui, E., Samuel, B., Coggins, J., McLeod, R., & Roberts, C. (2004). A complete shikimate pathway in Toxoplasma gondii: an ancient eukaryotic innovation. International Journal for Parasitology, 34(1), 5-13. https://doi.org/10.1016/j.ijpara.2003.10.006

• Journal Article Type: Article
• Acceptance Date: Oct 16, 2003
• Online Publication Date: Nov 26, 2003
• Publication Date: 2004-01
• Deposit Date: May 11, 2016
• Print ISSN: 0020-7519
• Electronic ISSN: 1879-0135
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 34
• Issue: 1
• Pages: 5-13
• DOI: https://doi.org/10.1016/j.ijpara.2003.10.006
• Keywords: Apicomplexa; Toxoplasma; Plasmodium; Shikimate; AROM; DAHP synthase
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/10147
• Publisher URL: http://dx.doi.org/10.1016/j.ijpara.2003.10.006