A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group

Stepanovi?, Srdjan; Daki?, Ivana; Martel, An; Vaneechoutte, Mario; Morrison, Donald; Shittu, Adebayo; Je�ek, Petr; Decostere, Annemie; Devriese, Luc A; Haesebrouck, Freddy

Abstract

Members of the Staphylococcus sciuri group (S. sciuri, S. lentus, and S. vitulinus) are coagulase-negative, novobiocin-resistant staphylococci that could be distinguished from other staphylococci on the basis of positive oxidase activity. In the present study, a scheme based on conventional methods and utilization of various carbohydrates was evaluated for the identification of oxidase-positive staphylococci, and validated using two molecular techniques. Of the 173 oxidase-positive staphylococcal tested strains, 161 were identified as S. sciuri, 9 as S. lentus, 2 as S. vitulinus, and one as S. fleurettii by our scheme. The level of agreement with tRNA intergenic length polymorphism analysis (tDNA-PCR) was high (97.5–100% correlation). The accuracy and ease of use of this protocol suggest that it may be useful and valuable in microbiological laboratories for the identification of members of this group.

Citation

Stepanović, S., Dakić, I., Martel, A., Vaneechoutte, M., Morrison, D., Shittu, A., Ježek, P., Decostere, A., Devriese, L. A., & Haesebrouck, F. (2005). A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group. Systematic and Applied Microbiology, 28(4), 353-357. https://doi.org/10.1016/j.syapm.2005.02.001

• Journal Article Type: Article
• Online Publication Date: Mar 3, 2005
• Publication Date: 2005-06
• Deposit Date: Jun 12, 2015
• Print ISSN: 0723-2020
• Electronic ISSN: 1618-0984
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 28
• Issue: 4
• Pages: 353-357
• DOI: https://doi.org/10.1016/j.syapm.2005.02.001
• Keywords: Staphylococcus sciuri; Staphylococcus lentus; Staphylococcus vitulinus; Staphylococcus fleurettii; ; Biochemical characteristic; Identification; tDNA-PCR
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/8679
• Publisher URL: http://dx.doi.org/10.1016/j.syapm.2005.02.001