Identification and molecular characterization of mannitol salt positive, coagulase-negative staphylococci from nasal samples of medical personnel and students.

Shittu, Adebayo; Lin, Johnson; Morrison, Donald; Kolawole, Deboye

Abstract

The identification of mannitol salt positive, coagulase-negative staphylococci (CNS) is often disregarded when Staphylococcus aureus is screened in clinical samples using mannitol salt agar. However, the emergence of CNS as important human pathogens has indicated that reliable methods for the identification of clinically significant CNS are of great importance in understanding the epidemiology of infections caused by them. The identification and molecular characterization of mannitol salt positive CNS from nasal samples of medical personnel and students is reported here. A total of 84 mannitol salt positive staphylococcal isolates were obtained from 240 nasal samples, of which 15 were CNS. The API STAPH system classified the CNS isolates into six species, and one-third of the isolates were identified with confidence levels of

Citation

Shittu, A., Lin, J., Morrison, D., & Kolawole, D. (2006). Identification and molecular characterization of mannitol salt positive, coagulase-negative staphylococci from nasal samples of medical personnel and students. Journal of Medical Microbiology, 55, 317-324. https://doi.org/10.1099/jmm.0.46072-0

• Journal Article Type: Article
• Publication Date: 2006
• Deposit Date: Jun 12, 2015
• Print ISSN: 0022-2615
• Electronic ISSN: 1473-5644
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 55
• Pages: 317-324
• DOI: https://doi.org/10.1099/jmm.0.46072-0
• Keywords: CNS; coagulase-negative staphylococci; ITS-PCR; 16S–23S rRNA intergenic spacer length polymorphism analysis; MSA; mannitol salt agar
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/8688
• Publisher URL: http://dx.doi.org/10.1099/jmm.0.46072-0