Physicochemical characterisation of cellulose from perennial ryegrass leaves (Lolium perenne).

Ren, J.L.; Sun, R.C.; Fowler, P.; Liu, C.F.; Xu, F.; Sun, J.X.; Ren, S; Curling, Simon; Sun, P; Fowler, P A; Baird, M.S.

Abstract

In this study, we investigated the physicochemical properties of the cellulosic preparations obtained from both untreated perennial ryegrass leaves and de-juiced leaves. It was found that treatment at 22 degrees C with 18% NaOH and 18% KOH for 2h, and 10% NaOH and 10% KOH for 16 h yielded 28.2%, 28.8%, 22.7%, 23.4%, respectively, of 'cellulose' residue from untreated ryegrass leaves and 35.7%, 36.8%, 32.8% and 34.6%, respectively, from the de-juiced leaves. For each cellulosic fraction, the glucose content was 71.6%, 69.6%, 67.8%, 66.7%, 69.7%, 68.6%, 63.9% and 61.7%, respectively. The structure of the cellulose samples was examined using FTIR and CP/MAS (13)C NMR spectroscopy and X-ray diffraction. The cellulosic preparations were free of bound lignin except for noticeable amounts of residual hemicelluloses (28.4-38.3%), and had intrinsic viscosities between 275.1 and 361.0 mL/g, along with molecular weights from 144,130 to 194,930 g/mol. This study found that the cellulose samples isolated from both de-juiced ryegrass leaves and the untreated leaves had a much lower percent crystallinity (33.0-38.6%) than that from wood-based fibres (60-70%) and had much shorter fibres (0.35-0.49 mm) than those of either cereal straws, bagasse or wood. In addition, a partial disruption of the hydrogen bonds and microfibrils may occur during the de-juicing process by mechanical activity, which results in a decreased cellulose crystallinity and fibre length. These findings are significant in relation to hydrolysing ryegrass cellulose for bio-ethanol production.

Citation

Ren, J., Sun, R., Fowler, P., Liu, C., Xu, F., Sun, J., Ren, S., Curling, S., Sun, P., Fowler, P. A., & Baird, M. (2006). Physicochemical characterisation of cellulose from perennial ryegrass leaves (Lolium perenne). Carbohydrate Research, 341(16), 2677-2687. https://doi.org/10.1016/j.carres.2006.07.008

• Journal Article Type: Article
• Publication Date: Aug 24, 2006
• Deposit Date: Jun 9, 2008
• Print ISSN: 0008-6215
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 341
• Issue: 16
• Pages: 2677-2687
• DOI: https://doi.org/10.1016/j.carres.2006.07.008
• Keywords: physicochemical; cellulose; ryegrass leaves; bio-ethanol production
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/1990
• Publisher URL: http://dx.doi.org/10.1016/j.carres.2006.07.008