The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line.

Clift, Martin James David; Rothen-Rutishauer, Barbara; Brown, David M; Duffin, Rodger; Donaldson, Ken; Proudfoot, Lorna; Guy, Keith; Stone, Vicki

Abstract

This study investigated the uptake, kinetics and cellular distribution of different surface coated quantum dots (QDs) before relating this to their toxicity. J774.A1 cells were treated with organic, COOH and NH2 (PEG) surface coated QDs (40 nM). Model 20 nm and 200 nm COOH-modified coated polystyrene beads (PBs) were also examined (50 microg ml(-1)). The potential for uptake of QDs was examined by both fixed and live cell confocal microscopy as well as by flow cytometry over 2 h. Both the COOH 20 nm and 200 nm PBs were clearly and rapidly taken up by the J774.A1 cells, with uptake of 20 nm PBs being relatively quicker and more extensive. Similarly, COOH QDs were clearly taken up by the macrophages. Uptake of NH2 (PEG) QDs was not detectable by live cell imaging however, was observed following 3D reconstruction of fixed cells, as well as by flow cytometry. Cells treated with organic QDs, monitored by live cell imaging, showed only a small amount of uptake in a relatively small number of cells. This uptake was insufficient to be detected by flow cytometry. Imaging of fixed cells was not possible due to a loss in cell integrity related to cytotoxicity. A significant reduction (p

Citation

Clift, M. J. D., Rothen-Rutishauer, B., Brown, D. M., Duffin, R., Donaldson, K., Proudfoot, L., …Stone, V. (2008). The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line. Toxicology and Applied Pharmacology, 232, 418-427. https://doi.org/10.1016/j.taap.2008.06.009

• Journal Article Type: Article
• Publication Date: 2008
• Deposit Date: Jun 22, 2012
• Print ISSN: 0041-008X
• Electronic ISSN: 0041-008X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 232
• Pages: 418-427
• DOI: https://doi.org/10.1016/j.taap.2008.06.009
• Keywords: Surface coating; Quantum dots (QDs); Polystyrene beads (PBs); J774.A1 macrophages; Nanoparticles (NPs); Confocal microscopy; Cellular uptake; Fluorescent stability; Toxicity
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/5478
• Publisher URL: http://dx.doi.org/10.1016/j.taap.2008.06.009