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Comparative assessment of a DNA and protein Leishmania donovani gamma glutamyl cysteine synthetase vaccine to cross-protect against murine cutaneous leishmaniasis caused by L. major or L. mexicana infection

Campbell, S.A.; Campbell, Samantha A; Alawa, J.; Doro, B.; Henriquez, F.L.; Roberts, C.W.; Nok, A.; Alawa, C.B.I.; Alsaadi, M.; Mullen, A.B.; Carter, K.C.

Authors

S.A. Campbell

J. Alawa

B. Doro

F.L. Henriquez

C.W. Roberts

A. Nok

C.B.I. Alawa

M. Alsaadi

A.B. Mullen

K.C. Carter



Abstract

Leishmaniasis is a major health problem and it is estimated that 12 million people are currently infected. A vaccine which could cross-protect people against different Leishmania spp. would facilitate control of this disease as more than one species of Leishmania may be present. In this study the ability of a DNA vaccine, using the full gene sequence for L. donovani gamma glutamyl cysteine synthetase (γGCS) incorporated in the pVAX vector (pVAXγGCS), and a protein vaccine, using the corresponding recombinant L. donovani γGCS protein (LdγGCS), to protect against L. major or L. mexicana infection was evaluated. DNA vaccination gave transient protection against L. major and no protection against L. mexicana despite significantly enhancing specific antibody titres in vaccinated infected mice compared to infected controls. Vaccination with the LdγGCS protected against both species but only if the protein was incorporated into non-ionic surfactant vesicles for L. mexicana. The results of this study indicate that a L. donovani γGCS vaccine could be used to vaccinate against more than one Leishmania species but only if the recombinant protein is used.

Journal Article Type Article
Acceptance Date Dec 13, 2011
Online Publication Date Dec 28, 2011
Publication Date Feb 8, 2012
Deposit Date May 10, 2016
Journal Vaccine
Print ISSN 0264-410X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 30
Issue 7
Pages 1357-1363
DOI https://doi.org/10.1016/j.vaccine.2011.12.067
Keywords Gamma glutamyl cysteine synthetase; Leishmania; Vaccine;
Public URL http://researchrepository.napier.ac.uk/id/eprint/10131
Publisher URL http://dx.doi.org/10.1016/j.vaccine.2011.12.067
Contract Date May 10, 2016