Skip to main content

Research Repository

Advanced Search

Isolation and characterization of soilborne virulent bacteriophages infecting the pathogen Rhodococcus equi

Salifu, S.P.; Casey, S.A. Campbell; Foley, S.


S.P. Salifu


To isolate and characterize a diversity of bacteriophages (phages) that infect the soilborne pathogen Rhodococcus equi.
Methods and Results
Twenty-seven phages were isolated from soil samples from geographically distinct locations using a range of R. equi bacterial strains, including clinical isolates. On the basis of host range, genomic DNA restriction profiles and virion protein profiles, the diversity of these phages was extensive, with phages being divided into 16 groupings.
Based on a range of criteria, these phages could be divided into 16 distinct groupings. The majority of the phages recovered from soil were Siphoviridae, adding to the limited number of Siphoviridae described to date for R. equi. One grouping consisted of phages belonging to the Myoviridae.
Significance and Impact of the Study
This represents the first study looking at the diversity of phages infecting the pathogen R. equi, including the first Myoviridae to be isolated and characterized for the genus Rhodococcus and for the nonmycobacterial actinomycetes. Given their diverse host range, including clinical isolates, this collection of phages offers the potential for the development of phage cocktails for use as a therapeutic or alternatively in the biocontrol of this pathogen in reservoirs of infection relating to animal husbandry.


Salifu, S., Casey, S. C., & Foley, S. (2013). Isolation and characterization of soilborne virulent bacteriophages infecting the pathogen Rhodococcus equi. Journal of Applied Microbiology, 114(6), 1625-1633.

Journal Article Type Article
Acceptance Date Mar 12, 2013
Online Publication Date Mar 16, 2013
Publication Date May 17, 2013
Deposit Date Mar 21, 2014
Journal Journal of Applied Microbiology
Print ISSN 1364-5072
Electronic ISSN 1365-2672
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 114
Issue 6
Pages 1625-1633
Keywords bacteriophage(s); diversity; Rhodococcus equi
Public URL
Publisher URL