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The effect of concentration and duration of normobaric oxygen in reducing caspase-3 and -9 expression in a rat-model of focal cerebral ischaemia

Chen, Suyan; Peng, Huizhen; Rowat, Anne; Gao, Feng; Zhang, Zhenxiang; Wang, Peng; Zhang, Weihong; Wang, Xianyuan; Qu, Lixia

Authors

Suyan Chen

Huizhen Peng

Anne Rowat

Feng Gao

Zhenxiang Zhang

Peng Wang

Weihong Zhang

Xianyuan Wang

Lixia Qu



Abstract

The aim of this study was to determine the effect of different concentrations of normobaric oxygen (NBO) on neurological function and the expression of caspase-3 and -9 in a rat model of acute cerebral ischaemia. Sprague-Dawley rats (n=120) were randomly divided into four groups (n=30 per group), including 3 groups given NBO at concentrations of 33%, 45% or 61% and one control group given air (21% oxygen). After 2 h of ischaemic occlusion, each group was further subdivided into six subgroups (n=5) during reperfusion according to the duration (3, 6, 12, 24, 48 or 72 h) and concentration of NBO (33%, 45% or 61%) or air treatment. The Fluorescence Quantitative polymerase chain reaction (PCR) and immunohistochemistry were used to detect caspase-3 and -9 mRNA and protein relative expression respectively. The Neurologic Impairment Score (NIS) was significantly lower in rats given 61% NBO ≥3 h after reperfusion when compared to the control group (P<0.05, Mann–Whitney U). NBO significantly reduced caspase-3 and -9 mRNA and protein expression when compared to the control group at all NBO concentrations and time points (P<0.05, ANOVA). The expression of caspase-3 and -9 was lower in the group given 61% NBO compared any other group, and this difference was statistically significant when compared to the group given 33% NBO for ≥48 h and the control group (both P<0.05, ANOVA). These findings indicate that NBO may inhibit the apoptotic pathway by reducing caspase-3 and -9 expression, thereby promoting neurological functional recovery after stroke.

Journal Article Type Article
Acceptance Date May 22, 2015
Online Publication Date May 30, 2015
Publication Date 2015-08
Deposit Date Jun 5, 2015
Publicly Available Date May 31, 2016
Journal Brain Research
Print ISSN 0006-8993
Electronic ISSN 1872-6240
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1618
Pages 205-211
DOI https://doi.org/10.1016/j.brainres.2015.05.027
Keywords Normobaric oxygen; Ischaemic rat model; Cerebral ischaemic–reperfusion; Apoptosis;
Public URL http://researchrepository.napier.ac.uk/id/eprint/8562
Publisher URL http://dx.doi.org/10.1016/j.brainres.2015.05.027
Contract Date Jun 5, 2015

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