The Ex Vivo Organ Culture of Bone

Staines, Katherine A.; Brown, Genevieve; Farquharson, Colin

Abstract

The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skeletal growth and development. In this chapter, we outline the basic protocols and possible readouts of organ culture models to replicate; (a) linear bone growth (murine metatarsal culture model), (b) bone and cartilage metabolism (murine femoral head culture model), (c) bone response to mechanical stimulation (bovine trabecular core culture model), and (d) bone resorption and formation (murine calvaria culture model).

Citation

Staines, K. A., Brown, G., & Farquharson, C. (2019). The Ex Vivo Organ Culture of Bone. In Bone Research Protocols (199-215). New york, USA: Humana Press. https://doi.org/10.1007/978-1-4939-8997-3_10

• Acceptance Date: Feb 8, 2019
• Online Publication Date: Feb 8, 2019
• Publication Date: 2019
• Deposit Date: Feb 13, 2019
• Journal: Methods in Molecular Biology; Bone Research Protocols
• Print ISSN: 1064-3745
• Electronic ISSN: 1940-6029
• Publisher: Humana Press
• Pages: 199-215
• Series Title: Methods in Molecular Biology
• Series Number: 1914
• Series ISSN: 1064-3745
• Book Title: Bone Research Protocols
• ISBN: 9781493989966
• DOI: https://doi.org/10.1007/978-1-4939-8997-3_10
• Keywords: Metatarsal, calvaria, femoral head, Trabecular core, organ culture,
• Public URL: http://researchrepository.napier.ac.uk/Output/1579072