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The Ex Vivo Organ Culture of Bone

Staines, Katherine A.; Brown, Genevieve; Farquharson, Colin


Katherine A. Staines

Genevieve Brown

Colin Farquharson


The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skeletal growth and development. In this chapter, we outline the basic protocols and possible readouts of organ culture models to replicate; (a) linear bone growth (murine metatarsal culture model), (b) bone and cartilage metabolism (murine femoral head culture model), (c) bone response to mechanical stimulation (bovine trabecular core culture model), and (d) bone resorption and formation (murine calvaria culture model).


Staines, K. A., Brown, G., & Farquharson, C. (2019). The Ex Vivo Organ Culture of Bone. In Bone Research Protocols (199-215). New york, USA: Humana Press.

Acceptance Date Feb 8, 2019
Online Publication Date Feb 8, 2019
Publication Date 2019
Deposit Date Feb 13, 2019
Journal Methods in Molecular Biology; Bone Research Protocols
Print ISSN 1064-3745
Electronic ISSN 1940-6029
Publisher Humana Press
Pages 199-215
Series Title Methods in Molecular Biology
Series Number 1914
Series ISSN 1064-3745
Book Title Bone Research Protocols
ISBN 9781493989966
Keywords Metatarsal, calvaria, femoral head, Trabecular core, organ culture,
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