The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

Boyd, Gary William; Drew, Marion; Ward, Shannon; Baird, Marianne; Graham, Scott Murray; Connaboy, Christopher

Abstract

Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used “sports”/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki’ = 24.9mM) and L-valine, the least potent (Ki’ = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.

Citation

Boyd, G. W., Drew, M., Ward, S., Baird, M., Graham, S. M., & Connaboy, C. (2018). The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase. Applied Physiology, Nutrition, and Metabolism, https://doi.org/10.1139/apnm-2018-0449

• Journal Article Type: Article
• Acceptance Date: Oct 14, 2018
• Online Publication Date: Nov 6, 2018
• Publication Date: Nov 8, 2018
• Deposit Date: Jan 29, 2019
• Publicly Available Date: Jan 30, 2019
• Journal: Applied Physiology, Nutrition, and Metabolism
• Print ISSN: 1715-5312
• Electronic ISSN: 1715-5320
• Publisher: NRC Research Press
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1139/apnm-2018-0449
• Keywords: Nutrition and Dietetics; Physiology (medical); Physiology; Endocrinology, Diabetes and Metabolism; General Medicine
• Public URL: http://researchrepository.napier.ac.uk/Output/1540850
• Contract Date: Jan 30, 2019