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The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

Boyd, Gary William; Drew, Marion; Ward, Shannon; Baird, Marianne; Graham, Scott Murray; Connaboy, Christopher

Authors

Gary William Boyd

Marion Drew

Shannon Ward

Marianne Baird

Scott Murray Graham

Christopher Connaboy



Abstract

Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used “sports”/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki’ = 24.9mM) and L-valine, the least potent (Ki’ = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.

Citation

Boyd, G. W., Drew, M., Ward, S., Baird, M., Graham, S. M., & Connaboy, C. (2018). The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase. Applied Physiology, Nutrition, and Metabolism, https://doi.org/10.1139/apnm-2018-0449

Journal Article Type Article
Acceptance Date Oct 14, 2018
Online Publication Date Nov 6, 2018
Publication Date Nov 8, 2018
Deposit Date Jan 29, 2019
Publicly Available Date Jan 30, 2019
Journal Applied Physiology, Nutrition, and Metabolism
Print ISSN 1715-5312
Electronic ISSN 1715-5320
Publisher NRC Research Press
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1139/apnm-2018-0449
Keywords Nutrition and Dietetics; Physiology (medical); Physiology; Endocrinology, Diabetes and Metabolism; General Medicine
Public URL http://researchrepository.napier.ac.uk/Output/1540850
Contract Date Jan 30, 2019

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