Outputs (39)

The SAbyNA platform: a guidance tool to support industry in the implementation of safe- and sustainable-by-design concepts for nanomaterials, processes and nano-enabled products (2025)
Journal Article
Cazzagon, V., Vanhauten, R., Hanlon, J., Sánchez Jiménez, A., Harrison, S., Auffan, M., Braakhuis, H., Boyles, M., Candalija, A., Katsumiti, A., Rodriguez-Llopis, I., Catalan, J., Cross, R. K., Lahive, E., Morel, E., Simeone, F. C., Delpivo, C., Clavaguera, S., Seddon, R., Salmatonidis, A., …Vázquez-Campos, S. (online). The SAbyNA platform: a guidance tool to support industry in the implementation of safe- and sustainable-by-design concepts for nanomaterials, processes and nano-enabled products. Environmental Science: Nano, https://doi.org/10.1039/d5en00312a

Simple, cost-effective and reliable methods are needed for pragmatic, flexible, safe and sustainable evaluations at early stages of product (chemical/material) development. This is especially true for nanoforms and nano-enabled products, for which gu... Read More about The SAbyNA platform: a guidance tool to support industry in the implementation of safe- and sustainable-by-design concepts for nanomaterials, processes and nano-enabled products.

Application of in vitro pulmonary models for hazard screening of silica particles (2025)
Journal Article
Ruijter, N., Braakhuis, H., Katsumiti, A., Garriz, I. P., Carriere, M., Zanoni, I., Candalija, A., Marshall, J., Vermeulen, J., Cassee, F. R., & Boyles, M. (online). Application of in vitro pulmonary models for hazard screening of silica particles. Archives of Toxicology, https://doi.org/10.1007/s00204-025-04100-5

There is an increasing need for new approach methodologies (NAMs) for safety assessment of nanomaterials (NMs) in order to keep pace with innovation. In vitro assays are useful tools during pre-market hazard screening approaches of NMs to prioritize... Read More about Application of in vitro pulmonary models for hazard screening of silica particles.

Hazard screening of colloidal silica nanomaterials with varying degrees of silane surface functionalization: a safe-by-design case study (2025)
Journal Article
Ruijter, N., Zanoni, I., Persson, D., Arts, J., Carriere, M., Guiot, A., Persson, M., Katsumiti, A., Marshall, J., Boyles, M., Cassee, F. R., & Braakhuis, H. (2025). Hazard screening of colloidal silica nanomaterials with varying degrees of silane surface functionalization: a safe-by-design case study. Particle and fibre toxicology, 22(1), Article 15. https://doi.org/10.1186/s12989-025-00629-6

Background: The Safe and Sustainable by Design (SSbD) concept facilitates the design of safer and more sustainable chemicals and materials and is a crucial approach towards reaching the goals set out in the European Green Deal. It is critical that su... Read More about Hazard screening of colloidal silica nanomaterials with varying degrees of silane surface functionalization: a safe-by-design case study.

The oxidative potential of nanomaterials: an optimized high-throughput protocol and interlaboratory comparison for the ferric reducing ability of serum (FRAS) assay (2024)
Journal Article
Ruijter, N., Boyles, M., Braakhuis, H., Ayerbe Algaba, R., Lofty, M., di Battista, V., Wohlleben, W., Cassee, F. R., & Candalija, A. (2024). The oxidative potential of nanomaterials: an optimized high-throughput protocol and interlaboratory comparison for the ferric reducing ability of serum (FRAS) assay. Nanotoxicology, 18(8), 724-738. https://doi.org/10.1080/17435390.2024.2438116

Successful implementation of Safe and Sustainable by Design (SSbD) and grouping approaches requires simple, reliable, and cost-effective assays to facilitate hazard screening at early stages of product development. Especially for nanomaterials (NMs),... Read More about The oxidative potential of nanomaterials: an optimized high-throughput protocol and interlaboratory comparison for the ferric reducing ability of serum (FRAS) assay.

Investigating Exposure and Hazards of Micro- and Nanoplastics During Pregnancy and Early Life (AURORA Project): Protocol for an Interdisciplinary Study (2024)
Journal Article
Durkin, A. M., Zou, R., Boucher, J. M., Boyles, M. S., van Boxel, J., Bustamante, M., Christopher, E. A., Dadvand, P., Dusza, H. M., van Duursen, M., Forsberg, M. M., Galea, K. S., Legler, J., Mandemaker, L. D., Meirer, F., Muncke, J., Nawrot, T. S., Přibylová, P., Robuck, A. R., Saenen, N. D., …Vermeulen, R. (2024). Investigating Exposure and Hazards of Micro- and Nanoplastics During Pregnancy and Early Life (AURORA Project): Protocol for an Interdisciplinary Study. JMIR Research Protocols, 13, Article e63176. https://doi.org/10.2196/63176

Background:
Micro- and nanoplastics (MNPs) are emerging pollutants of concern with ubiquitous presence in global ecosystems. MNPs pose potential implications for human health; however, the health impacts of MNP exposures are not yet understood. Rece... Read More about Investigating Exposure and Hazards of Micro- and Nanoplastics During Pregnancy and Early Life (AURORA Project): Protocol for an Interdisciplinary Study.

Safe(r)-by-design principles in the thermoplastics industry: guidance on release assessment during manufacture of nano-enabled products (2024)
Journal Article
McLean, P., Hanlon, J., Salmatonidis, A., Galea, K. S., Brooker, F., Citterio, C., Magni, D., Vázquez-Campos, S., Lotti, D., & Boyles, M. S. P. (2024). Safe(r)-by-design principles in the thermoplastics industry: guidance on release assessment during manufacture of nano-enabled products. Frontiers in Public Health, 12, https://doi.org/10.3389/fpubh.2024.1398104

Background: The application of nanomaterials (NMs) and nano-enabled products (NEPs) across many industries has been extensive and is still expanding decades after first being identified as an emerging technology. Additive manufacturing has been great... Read More about Safe(r)-by-design principles in the thermoplastics industry: guidance on release assessment during manufacture of nano-enabled products.

Impacts of micro- and nanoplastics on early-life health: a roadmap towards risk assessment (2024)
Journal Article
Christopher, E. A., Christopher-de Vries, Y., Devadoss, A., Mandemaker, L. D., van Boxel, J., Copsey, H. M., Dusza, H. M., Legler, J., Meirer, F., Muncke, J., Nawrot, T. S., Saenen, N. D., Scholz-Böttcher, B. M., Tran, L., Weckhuysen, B. M., Zou, R., Zimmermann, L., Galea, K. S., Vermeulen, R., & Boyles, M. S. P. (in press). Impacts of micro- and nanoplastics on early-life health: a roadmap towards risk assessment. Microplastics and Nanoplastics, 4(1), 13. https://doi.org/10.1186/s43591-024-00089-3

Micro- and nanoplastics (MNPs) are ubiquitous environmental pollutants representing a concern for human health. MNPs have been detected in human placentas, indicating that during pregnancy maternal exposure may lead to placental transfer and foetal e... Read More about Impacts of micro- and nanoplastics on early-life health: a roadmap towards risk assessment.

Improving the dichloro-dihydro-fluorescein (DCFH) assay for the assessment of intracellular reactive oxygen species formation by nanomaterials (2024)
Journal Article
Ruijter, N., van der Zee, M., Katsumiti, A., Boyles, M., Cassee, F. R., & Braakhuis, H. (2024). Improving the dichloro-dihydro-fluorescein (DCFH) assay for the assessment of intracellular reactive oxygen species formation by nanomaterials. NanoImpact, 35, Article 100521. https://doi.org/10.1016/j.impact.2024.100521

To facilitate Safe and Sustainable by Design (SSbD) strategies during the development of nanomaterials (NMs), quick and easy in vitro assays to test for hazard potential at an early stage of NM development are essential. The formation of reactive oxy... Read More about Improving the dichloro-dihydro-fluorescein (DCFH) assay for the assessment of intracellular reactive oxygen species formation by nanomaterials.

A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards (2024)
Journal Article
McLean, P., Marshall, J., García, A., Beal, D., Katsumiti, A., Carrière, M., & Boyles, M. (2024). A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards. Toxicology in Vitro, 97, Article 105792. https://doi.org/10.1016/j.tiv.2024.105792

The objective of Safe-by-Design (SbD) is to support the development of safer products and production processes, and enable safe use throughout a materials' life cycle; an intervention at an early stage of innovation can greatly benefit industry by re... Read More about A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards.

Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis (2023)
Journal Article
McLean, P., Mueller, W., Gosens, I., Cassee, F. R., Rothen-Rutishauser, B., Boyles, M., & Tran, L. (2023). Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis. Particle and fibre toxicology, 20(1), Article 5. https://doi.org/10.1186/s12989-023-00516-y

Background: Toxicity assessment for regulatory purposes is starting to move away from traditional in vivo methods and towards new approach methodologies (NAM) such as high-throughput in vitro models and computational tools. For materials with limited... Read More about Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis.