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Characterisation of autophagy induction by the thiopurine drugs azathioprine, mercaptopurine and thioguanine in THP-1 macrophages (2024)
Journal Article
Masson, C. D., Findlay-Greene, F., Henderson Sousa, F., Henderson, P., Fraser, J. A., Barlow, P. G., & Stevens, C. (online). Characterisation of autophagy induction by the thiopurine drugs azathioprine, mercaptopurine and thioguanine in THP-1 macrophages. Naunyn-Schmiedeberg's Archives of Pharmacology, https://doi.org/10.1007/s00210-024-03563-0

Purpose: Activating autophagy may be therapeutically beneficial, and we have previously shown that azathioprine (AZA), an immunomodulatory drug, induces autophagy. Here, we evaluated the induction of autophagy by the thiopurines AZA, mercaptopurine (... Read More about Characterisation of autophagy induction by the thiopurine drugs azathioprine, mercaptopurine and thioguanine in THP-1 macrophages.

Evolution and immunopathology of chikungunya virus informs therapeutic development (2023)
Journal Article
Henderson Sousa, F., Ghaisani Komarudin, A., Findlay-Greene, F., Bowolaksono, A., Sasmono, R. T., Stevens, C., & Barlow, P. G. (2023). Evolution and immunopathology of chikungunya virus informs therapeutic development. Disease Models and Mechanisms, 16(4), https://doi.org/10.1242/dmm.049804

Chikungunya virus (CHIKV), a mosquito-borne alphavirus, is an emerging global threat identified in more than 60 countries across continents. The risk of CHIKV transmission is rising due to increased global interactions, year-round presence of mosquit... Read More about Evolution and immunopathology of chikungunya virus informs therapeutic development.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) (2021)
Journal Article
Klionsky, D. J., Kamal Abdel-Aziz, A., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Stevens, C., & et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17(1), 1-382. https://doi.org/10.1080/15548627.2020.1797280

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have als... Read More about Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).

Models of osteoarthritis: relevance and new insights (2020)
Journal Article
Samvelyan, H., Hughes, D., Stevens, C., & Staines, K. A. (2021). Models of osteoarthritis: relevance and new insights. Calcified Tissue International, 109(3), 243-256. https://doi.org/10.1007/s00223-020-00670-x

Osteoarthritis (OA) is a progressive and disabling musculoskeletal disease affecting millions of people and resulting in major healthcare costs worldwide. It is the most common form of arthritis, characterised by degradation of the articular cartilag... Read More about Models of osteoarthritis: relevance and new insights.

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection (2020)
Journal Article
Casanova, V., Sousa, F. H., Shakamuri, P., Svoboda, P., Buch, C., D'Acremont, M., Christophorou, M. A., Pohl, J., Stevens, C., & Barlow, P. G. (2020). Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Frontiers in Immunology, 11, Article 85. https://doi.org/10.3389/fimmu.2020.00085

Human rhinoviruses (HRV) are the most common cause of viral respiratory tract infections. While normally mild and self-limiting in healthy adults, HRV infections are associated with bronchiolitis in infants, pneumonia in immunocompromised patients, a... Read More about Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection.

Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo (2019)
Journal Article
Mathur, S., Turnbull, A., Akaev, I., Stevens, C., Agrawal, N., Chopra, M., & Mincher, D. (2020). Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo. International Journal of Peptide Research and Therapeutics, 26, 1965-1980. https://doi.org/10.1007/s10989-019-09994-1

The lysosomal endoprotease legumain (asparaginyl endoprotease) has been proposed as a putative biomarker in prostate tumours, in which the enzyme is markedly overexpressed. Overexpression, coupled with highly selective specificity for cleavage of sub... Read More about Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo.

Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease (2019)
Journal Article
Morgan, S., Hooper, K. M., Milne, E. M., Farquharson, C., Stevens, C., & Staines, K. A. (2019). Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease. International Journal of Molecular Sciences, 20(23), Article 6085. https://doi.org/10.3390/ijms20236085

Patients with inflammatory bowel disease (IBD) often present poor bone health and are 40% more at risk of bone fracture. Studies have implicated autophagy in IBD pathology and drugs used to treat IBD stimulate autophagy in varying degrees, however, t... Read More about Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease.

Regulation of the expression of DAPK1 by SUMO pathway. (2019)
Journal Article
Wang, Q., Zhang, X., Chen, L., Weng, S., Xia, Y., Ye, Y., Li, K., Liao, Z., Chen, P., Alsamman, K., Meng, C., Stevens, C., Hupp, T. R., & Lin, Y. (2019). Regulation of the expression of DAPK1 by SUMO pathway. Biomolecules, 9(4), 151. https://doi.org/10.3390/biom9040151

Death Associated Protein Kinase 1 (DAPK1) is an important signaling kinase mediating the biological e ect of multiple natural biomolecules such as IFN-, TNF- , curcumin, etc. DAPK1 is degraded through both ubiquitin-proteasomal and lysosomal degradat... Read More about Regulation of the expression of DAPK1 by SUMO pathway..

The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK (2019)
Journal Article
Hooper, K. M., Casanova, V., Kemp, S., Staines, K. A., Satsangi, J., Barlow, P. G., Henderson, P., & Stevens, C. (2019). The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK. Inflammatory Bowel Diseases, 25(9), 1481-1496. https://doi.org/10.1093/ibd/izz039

Background Genetic studies have strongly linked autophagy to Crohn's disease (CD) and stimulating autophagy in CD patients may be therapeutically beneficial. The aim of this study was to evaluate the effect of current inflammatory bowel disease (IBD)... Read More about The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK.