All Outputs (32)

A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards (2024)
Journal Article
McLean, P., Marshall, J., García, A., Beal, D., Katsumiti, A., Carrière, M., & Boyles, M. (2024). A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards. Toxicology in Vitro, 97, Article 105792. https://doi.org/10.1016/j.tiv.2024.105792

The objective of Safe-by-Design (SbD) is to support the development of safer products and production processes, and enable safe use throughout a materials' life cycle; an intervention at an early stage of innovation can greatly benefit industry by re... Read More about A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards.

Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis (2023)
Journal Article
McLean, P., Mueller, W., Gosens, I., Cassee, F. R., Rothen-Rutishauser, B., Boyles, M., & Tran, L. (2023). Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis. Particle and fibre toxicology, 20(1), Article 5. https://doi.org/10.1186/s12989-023-00516-y

Background: Toxicity assessment for regulatory purposes is starting to move away from traditional in vivo methods and towards new approach methodologies (NAM) such as high-throughput in vitro models and computational tools. For materials with limited... Read More about Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis.

The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design (2023)
Journal Article
Ruijter, N., Soeteman-Hernández, L. G., Carrière, M., Boyles, M., McLean, P., Catalán, J., …Braakhuis, H. (2023). The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design. Nanomaterials, 13(3), Article 472. https://doi.org/10.3390/nano13030472

The Safe-by-Design (SbD) concept aims to facilitate the development of safer materials/products, safer production, and safer use and end-of-life by performing timely SbD interventions to reduce hazard, exposure, or both. Early hazard screening is a c... Read More about The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.

Integrated approaches to testing and assessment for grouping nanomaterials following dermal exposure (2022)
Journal Article
Di Cristo, L., Janer, G., Dekkers, S., Boyles, M., Giusti, A., Keller, J. G., …Sabella, S. (2022). Integrated approaches to testing and assessment for grouping nanomaterials following dermal exposure. Nanotoxicology, 16(3), 310-332. https://doi.org/10.1080/17435390.2022.2085207

Exposure to different nanoforms (NFs) via the dermal route is expected in occupational and consumer settings and thus it is important to assess their dermal toxicity and the contribution of dermal exposure to systemic bioavailability. We have formula... Read More about Integrated approaches to testing and assessment for grouping nanomaterials following dermal exposure.

Determining nanoform similarity via assessment of surface reactivity by abiotic and in vitro assays (2022)
Journal Article
Ag Seleci, D., Tsiliki, G., Werle, K., Elam, D. A., Okpowe, O., Seidel, K., …Wohlleben, W. (2022). Determining nanoform similarity via assessment of surface reactivity by abiotic and in vitro assays. NanoImpact, 26, Article 100390. https://doi.org/10.1016/j.impact.2022.100390

Grouping of substances is a method used to streamline hazard and risk assessment. Assessment of similarity provides the scientific evidence needed for formation of groups. This work reports on justification of grouping of nanoforms (NFs) via similari... Read More about Determining nanoform similarity via assessment of surface reactivity by abiotic and in vitro assays.

Bayesian based similarity assessment of nanomaterials to inform grouping (2022)
Journal Article
Tsiliki, G., Ag Seleci, D., Zabeo, A., Basei, G., Hristozov, D., Jeliazkova, N., …Stone, V. (2022). Bayesian based similarity assessment of nanomaterials to inform grouping. NanoImpact, 25, Article 100389. https://doi.org/10.1016/j.impact.2022.100389

Nanoforms can be manufactured in plenty of variants by differing their physicochemical properties and toxicokinetic behaviour which can affect their hazard potential. To avoid testing of each single nanomaterial and nanoform variation and subsequentl... Read More about Bayesian based similarity assessment of nanomaterials to inform grouping.

Development of a standard operating procedure for the DCFH2-DA acellular assessment of reactive oxygen species produced by nanomaterials (2022)
Journal Article
Boyles, M., Murphy, F., Mueller, W., Wohlleben, W., Jacobsen, N. R., Braakhuis, H., …Stone, V. (2022). Development of a standard operating procedure for the DCFH2-DA acellular assessment of reactive oxygen species produced by nanomaterials. Toxicology Mechanisms and Methods, 32(6), 439-452. https://doi.org/10.1080/15376516.2022.2029656

Improved strategies are required for testing nanomaterials (NMs) to make hazard and risk assessment more efficient and sustainable. Including reduced reliance on animal models, without decreasing the level of human health protection. Acellular detect... Read More about Development of a standard operating procedure for the DCFH2-DA acellular assessment of reactive oxygen species produced by nanomaterials.

Paradigms to assess the human health risks of nano- and microplastics (2021)
Journal Article
Noventa, S., Boyles, M. S. P., Seifert, A., Belluco, S., Jiménez, A. S., Johnston, H. J., …Losasso, C. (2021). Paradigms to assess the human health risks of nano- and microplastics. Microplastics and Nanoplastics, 1, Article 9. https://doi.org/10.1186/s43591-021-00011-1

Human exposure to nano- and microplastics (NMPs) has raised major societal concerns, yet no framework to assess the risks of NMPs for human health exists. A substantial proportion of plastic produced worldwide is not properly disposed and persists in... Read More about Paradigms to assess the human health risks of nano- and microplastics.

Simulated biological fluids – a systematic review of their biological relevance and use in relation to inhalation toxicology of particles and fibres (2021)
Journal Article
Innes, E., Yiu, H. H. P., McLean, P., Brown, W., & Boyles, M. (2021). Simulated biological fluids – a systematic review of their biological relevance and use in relation to inhalation toxicology of particles and fibres. Critical Reviews in Toxicology, 51(3), 217-248. https://doi.org/10.1080/10408444.2021.1903386

The use of simulated biological fluids (SBFs) is a promising in vitro technique to better understand the release mechanisms and possible in vivo behaviour of materials, including fibres, metal-containing particles and nanomaterials. Applications of S... Read More about Simulated biological fluids – a systematic review of their biological relevance and use in relation to inhalation toxicology of particles and fibres.

A Method to Assess the Relevance of Nanomaterial Dissolution during Reactivity Testing (2020)
Journal Article
Peijnenburg, W. J. G. M., Ruggiero, E., Boyles, M., Murphy, F., Stone, V., Elam, D. A., …Wohlleben, W. (2020). A Method to Assess the Relevance of Nanomaterial Dissolution during Reactivity Testing. Materials, 13(10), Article 2235. https://doi.org/10.3390/ma13102235

The reactivity of particle surfaces can be used as a criterion to group nanoforms (NFs) based on similar potential hazard. Since NFs may partially or completely dissolve over the duration of the assays, with the ions themselves inducing a response, r... Read More about A Method to Assess the Relevance of Nanomaterial Dissolution during Reactivity Testing.

Assessment of the physicochemical properties of chrysotile-containing brake debris pertaining to toxicity (2019)
Journal Article
Boyles, M. S. P., Poland, C. A., Raftis, J., & Duffin, R. (2019). Assessment of the physicochemical properties of chrysotile-containing brake debris pertaining to toxicity. Inhalation Toxicology, 31(8), 325-342. https://doi.org/10.1080/08958378.2019.1683103

Grinding and drilling of chrysotile asbestos-containing brake pads during the 20thcentury led torelease of chrysotile, resulting in varying levels of workplace exposures of mechanics. Despite expo-sures, excess risk of mesothelioma remains in doubt.... Read More about Assessment of the physicochemical properties of chrysotile-containing brake debris pertaining to toxicity.

Assessing the bioactivity of crystalline silica in heated high-temperature insulation wools (2018)
Journal Article
Boyles, M. S. P., Brown, D., Knox, J., Horobin, M., Miller, M. R., Johnston, H. J., & Stone, V. (2018). Assessing the bioactivity of crystalline silica in heated high-temperature insulation wools. Inhalation Toxicology, 30(7-8), 255-272. https://doi.org/10.1080/08958378.2018.1513610

High-Temperature Insulation Wools (HTIW), such as alumino silicate wools (Refractory Ceramic Fibers) and Alkaline Earth Silicate wools, are used in high-temperature industries for thermal insulation. These materials have an amorphous glass-like struc... Read More about Assessing the bioactivity of crystalline silica in heated high-temperature insulation wools.

Releases from transparent blue automobile coatings containing nanoscale copper phthalocyanine and their effects on J774 A1 macrophages (2017)
Journal Article
Pang, C., Neubauer, N., Boyles, M., Brown, D., Kanase, N., Hristozov, D., …Marcomini, A. (2017). Releases from transparent blue automobile coatings containing nanoscale copper phthalocyanine and their effects on J774 A1 macrophages. NanoImpact, 7, 75-83. https://doi.org/10.1016/j.impact.2017.06.002

Nanoscale copper phthalocyanine (n-CuPc) is a pigment widely used in paints to enhance automobile coatings and to make colors transparent and more appealing to users. Despite the benefits, n-CuPc can potentially be released into the environment and p... Read More about Releases from transparent blue automobile coatings containing nanoscale copper phthalocyanine and their effects on J774 A1 macrophages.

A Novel Exposure System Termed NAVETTA for In Vitro Laminar Flow Electrodeposition of Nanoaerosol and Evaluation of Immune Effects in Human Lung Reporter Cells (2017)
Journal Article
Frijns, E., Verstraelen, S., Stoehr, L. C., Van Laer, J., Jacobs, A., Peters, J., …Himly, M. (2017). A Novel Exposure System Termed NAVETTA for In Vitro Laminar Flow Electrodeposition of Nanoaerosol and Evaluation of Immune Effects in Human Lung Reporter Cells. Environmental Science and Technology, 51(9), 5259-5269. https://doi.org/10.1021/acs.est.7b00493

A new prototype air–liquid interface (ALI) exposure system, a flatbed aerosol exposure chamber termed NAVETTA, was developed to investigate deposition of engineered nanoparticles (NPs) on cultured human lung A549 cells directly from the gas phase. Th... Read More about A Novel Exposure System Termed NAVETTA for In Vitro Laminar Flow Electrodeposition of Nanoaerosol and Evaluation of Immune Effects in Human Lung Reporter Cells.

Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts (2017)
Journal Article
da Luz, C. M., Boyles, M. S. P., Falagan-Lotsch, P., Pereira, M. R., Tutumi, H. R., de Oliveira Santos, E., …Leite, P. E. C. (2017). Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts. Journal of Nanobiotechnology, 15(1), Article 11. https://doi.org/10.1186/s12951-016-0238-1

Background Poly-lactic acid nanoparticles (PLA-NP) are a type of polymeric NP, frequently used as nanomedicines, which have advantages over metallic NP such as the ability to maintain therapeutic drug levels for sustained periods of time. Despite PL... Read More about Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts.

Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles (2016)
Journal Article
Piret, J., Bondarenko, O. M., Boyles, M. S. P., Himly, M., Ribeiro, A. R., Benetti, F., …Toussaint, O. (2017). Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles. Archives of Toxicology, 91(6), 2315-2330. https://doi.org/10.1007/s00204-016-1897-2

The rapid development of nanotechnologies and increased production and use of nanomaterials raise concerns about their potential toxic effects for human health and environment. To evaluate the biological effects of nanomaterials, a set of reliable an... Read More about Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles.

An Overview of Nanoparticle Biocompatibility for Their Use in Nanomedicine (2016)
Book Chapter
Boyles, M. S., Powell, L. G., Kermanizadeh, A., Johnston, H. J., Rothen-Rutishauser, B., Stone, V., & Clift, M. J. (2017). An Overview of Nanoparticle Biocompatibility for Their Use in Nanomedicine. In Pharmaceutical Nanotechnology: Innovation and Production: Innovation and Production (443-468). Wiley. https://doi.org/10.1002/9783527800681.ch18

Nanotechnology, considered an industrial revolution occurring across the twentieth and twenty first centuries, promises to provide numerous advantages to both humans and the environment, across consumer, industrial, and technology sectors. Although a... Read More about An Overview of Nanoparticle Biocompatibility for Their Use in Nanomedicine.

Copper oxide nanoparticle toxicity profiling using untargeted metabolomics (2016)
Journal Article
Boyles, M. S. P., Ranninger, C., Reischl, R., Rurik, M., Tessadri, R., Kohlbacher, O., …Huber, C. G. (2016). Copper oxide nanoparticle toxicity profiling using untargeted metabolomics. Particle and fibre toxicology, 13, Article 49. https://doi.org/10.1186/s12989-016-0160-6

Background The rapidly increasing number of engineered nanoparticles (NPs), and products containing NPs, raises concerns for human exposure and safety. With this increasing, and ever changing, catalogue of NPs it is becoming more difficult to adequa... Read More about Copper oxide nanoparticle toxicity profiling using untargeted metabolomics.

Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses (2016)
Journal Article
Radauer-Preiml, I., Andosch, A., Hawranek, T., Luetz-Meindl, U., Wiederstein, M., Horejs-Hoeck, J., …Duschl, A. (2016). Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses. Particle and fibre toxicology, 13, Article 3. https://doi.org/10.1186/s12989-016-0113-0

Engineered nanomaterials (ENMs) interact with different biomolecules as soon as they are in contact, resulting in the formation of a biomolecule ‘corona’. Hence, the ‘corona’ defines the biological identity of the ENMs and could affect the response o... Read More about Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses.

Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components (2015)
Journal Article
Boyles, M. S. P., Kristl, T., Andosch, A., Zimmermann, M., Tran, N., Casals, E., …Duschl, A. (2015). Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology, 13, Article 84. https://doi.org/10.1186/s12951-015-0146-9

Background Gold nanoparticles (AuNPs) are a popular choice for use in medical and biomedical research applications. With suitable functionalisation AuNPs can be applied in drug delivery systems, or can aid in disease diagnosis. One such functionalis... Read More about Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components.