Apoptosis induced by Paclitaxel, tobacco smoke condensate and hydrogen peroxide in lung epithelial cells (A549)

Ramage, Lindsay; Jones, Amanda; Whelan, Cliff

Abstract

Cigarette smoke is known to be a complex mixture of >4000 constituents dispersed between the volatile and non-volatile phase, and inhaled exposure causes lung inflammation and cell injury. Here we investigate the effects of some of components of cigarette smoke as inducers of apoptosis in A549 cells. Apoptosis is characterised by initial changes on the mitochondria, leading to chromatin condensation and ultimately DAM fragmentation. Cells were trated with 1um paclitaxel, 80ug/ml tobacco smoke condensate (TSC), 1uM nicotine and 10uM hydrogen peroxide for 24 and 48 hours. Attached and detached cells were stained with DAPI and visualised by fluoresence microscopy, % normal and apoptotic cells were determined. Results indicated morphological changes with time but quantification proved difficult. DNA was isolated and electrophoresed on 1% agarose gels. A DNA ladder was present in cells treated with paclitaxel, TSC and hydrogen peroxide but not nicotine. The presence of a DNA ladder indicates that late stage apoptotic events are occurring in these cells in exposure to certain components of tobacco smoke

• Presentation Conference Type: Conference Abstract
• Acceptance Date: Aug 1, 2004
• Publication Date: 2004-09
• Deposit Date: Jan 4, 2018
• Journal: Inflammation Research
• Print ISSN: 1023-3830
• Electronic ISSN: 1420-908X
• Publisher: BMC
• Peer Reviewed: Peer Reviewed
• Volume: 53
• Issue: S3
• Pages: S207-S230
• DOI: https://doi.org/10.1007/bf03353882
• Keywords: Immunology, Pharmacology
• Public URL: http://researchrepository.napier.ac.uk/Output/820821