Dr Sharron Vass S.Vass@napier.ac.uk
Lecturer
Perturbation of invadolysin disrupts cell migration in zebrafish (Danio rerio)
Vass, Sharron; Heck, Margarete M S
Authors
Margarete M S Heck
Abstract
Invadolysin is an essential, conserved metalloprotease which links cell division with cell migration and is intriguingly associated with lipid droplets. In this work we examine the expression pattern, protein localisation and gross anatomical consequences of depleting invadolysin in the teleost Danio rerio. We observe that invadolysin plays a significant role in cell migration during development. When invadolysin is depleted by targeted morpholino injection, the appropriate deposition of neuromast clusters and distribution of melanophores are both disrupted. We also observe that blood vessels generated via angiogenesis are affected in invadolysin morphant fish while those formed by vasculogenesis appear normal, demonstrating an unanticipated role for invadolysin in vessel formation. Our results thus highlight a common feature shared by, and a requirement for invadolysin in, these distinct morphological events dependent on cell migration.
Citation
Vass, S., & Heck, M. M. S. (2013). Perturbation of invadolysin disrupts cell migration in zebrafish (Danio rerio). Experimental Cell Research, 319(8), 1198-1212. https://doi.org/10.1016/j.yexcr.2013.02.005
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 2, 2013 |
Online Publication Date | Feb 16, 2013 |
Publication Date | 2013-05 |
Deposit Date | Sep 1, 2016 |
Publicly Available Date | Sep 19, 2017 |
Journal | Experimental Cell Research |
Print ISSN | 0014-4827 |
Electronic ISSN | 1090-2422 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 319 |
Issue | 8 |
Pages | 1198-1212 |
DOI | https://doi.org/10.1016/j.yexcr.2013.02.005 |
Keywords | Invadolysin; Metalloprotease; Cell migration; Vascular development; Zebrafish |
Public URL | http://researchrepository.napier.ac.uk/Output/369598 |
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Perturbation of invadolysin disrupts cell migration in zebrafish (Danio rerio)
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Publisher Licence URL
http://creativecommons.org/licenses/by/3.0/
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