Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family

Schofield, Peter R.; Darlison, Mark G.; Fujita, Norihisa; Burt, David R.; Stephenson, F. Anne; Rodriguez, Henry; Rhee, Lucy M.; Ramachandran, J.; Reale, Vincenzina; Glencorse, Thora A.; Seeburg, Peter H.; Barnard, Eric A.

Abstract

Amino-acid sequences derived from complementary DMAs encoding the α- and β-subunits of the GAB A/ benzo diazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated α-subunit and β-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.

Citation

Schofield, P. R., Darlison, M. G., Fujita, N., Burt, D. R., Stephenson, F. A., Rodriguez, H., Rhee, L. M., Ramachandran, J., Reale, V., Glencorse, T. A., Seeburg, P. H., & Barnard, E. A. (1987). Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature, 328(6127), 221-227. https://doi.org/10.1038/328221a0

• Journal Article Type: Article
• Acceptance Date: Jun 16, 1987
• Publication Date: Jul 16, 1987
• Deposit Date: Aug 5, 2016
• Journal: Nature
• Print ISSN: 0028-0836
• Electronic ISSN: 1476-4687
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 328
• Issue: 6127
• Pages: 221-227
• DOI: https://doi.org/10.1038/328221a0
• Keywords: Amino-acid sequences, DMAs encoding, GAB A/ benzo diazepine receptor, functional receptor channel,
• Public URL: http://researchrepository.napier.ac.uk/Output/327807