Evelien Frijns
A Novel Exposure System Termed NAVETTA for In Vitro Laminar Flow Electrodeposition of Nanoaerosol and Evaluation of Immune Effects in Human Lung Reporter Cells
Frijns, Evelien; Verstraelen, Sandra; Stoehr, Linda Corinna; Van Laer, Jo; Jacobs, An; Peters, Jan; Tirez, Kristof; Boyles, Matthew Samuel Powys; Geppert, Mark; Madl, Pierre; Nelissen, Inge; Duschl, Albert; Himly, Martin
Authors
Sandra Verstraelen
Linda Corinna Stoehr
Jo Van Laer
An Jacobs
Jan Peters
Kristof Tirez
Dr Matthew Boyles M.Boyles2@napier.ac.uk
Lecturer
Mark Geppert
Pierre Madl
Inge Nelissen
Albert Duschl
Martin Himly
Abstract
A new prototype air–liquid interface (ALI) exposure system, a flatbed aerosol exposure chamber termed NAVETTA, was developed to investigate deposition of engineered nanoparticles (NPs) on cultured human lung A549 cells directly from the gas phase. This device mimics human lung cell exposure to NPs due to a low horizontal gas flow combined with cells exposed at the ALI. Electrostatic field assistance is applied to improve NP deposition efficiency. As proof-of-principle, cell viability and immune responses after short-term exposure to nanocopper oxide (CuO)-aerosol were determined. We found that, due to the laminar aerosol flow and a specific orientation of inverted transwells, much higher deposition rates were obtained compared to the normal ALI setup. Cellular responses were monitored with postexposure incubation in submerged conditions, revealing CuO dissolution in a concentration-dependent manner. Cytotoxicity was the result of ionic and nonionic Cu fractions. Using the optimized inverted ALI/postincubation procedure, pro-inflammatory immune responses, in terms of interleukin (IL)-8 promoter and nuclear factor kappa B (NFκB) activity, were observed within short time, i.e. One hour exposure to ALI-deposited CuO-NPs and 2.5 h postincubation. NAVETTA is a novel option for mimicking human lung cell exposure to NPs, complementing existing ALI systems.
Citation
Frijns, E., Verstraelen, S., Stoehr, L. C., Van Laer, J., Jacobs, A., Peters, J., Tirez, K., Boyles, M. S. P., Geppert, M., Madl, P., Nelissen, I., Duschl, A., & Himly, M. (2017). A Novel Exposure System Termed NAVETTA for In Vitro Laminar Flow Electrodeposition of Nanoaerosol and Evaluation of Immune Effects in Human Lung Reporter Cells. Environmental Science and Technology, 51(9), 5259-5269. https://doi.org/10.1021/acs.est.7b00493
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 24, 2017 |
Online Publication Date | Apr 14, 2017 |
Publication Date | May 2, 2017 |
Deposit Date | Oct 13, 2023 |
Journal | Environmental Science & Technology |
Print ISSN | 0013-936X |
Electronic ISSN | 1520-5851 |
Publisher | American Chemical Society |
Peer Reviewed | Peer Reviewed |
Volume | 51 |
Issue | 9 |
Pages | 5259-5269 |
DOI | https://doi.org/10.1021/acs.est.7b00493 |
Public URL | http://researchrepository.napier.ac.uk/Output/3214045 |
PMID | 28339192 |
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