Senescent T-lymphocytes are mobilised into the peripheral blood compartment in young and older humans after exhaustive exercise

Simpson, Richard J; Cosgrove, Cormac; Ingram, Lesley A; Florida-James, Geraint D; Whyte, Greg P; Pircher, Hanspeter; Guy, Keith

Abstract

Senescent T-lymphocytes are antigen-experienced cells that express the killer-cell lectin-like receptor G1 (KLRG1) and/or CD57; fail to clonally expand following further antigenic stimulation and prevail in the resting blood of older adults compared to the young. Physical exercise mobilises T-lymphocytes into the bloodstream and is therefore a model with which to compare age-related phenotypes of blood-resident T-cells with those T-cells entering the blood from peripheral lymphoid compartments. Eight young (Y; Age: 21 ± 3 years) and 8 older (O; Age: 56 ± 3 years) healthy males completed a maximal treadmill exercise protocol. Blood lymphocytes isolated before, immediately after and 1 h after exercise were assessed for cell surface expression of KLRG1, CD57, CD28, CD45RA, CD45RO, CD62L and lymphocyte subset markers using three-colour flow cytometry. Lymphocyte subset numbers (CD3+, CD3+/CD4+, CD3+/CD8 and CD3−/CD56+) increased with exercise (p < 0.05) but were not different between Y and O. At rest and immediately after exercise, the percentage of CD3+/CD8+ T-lymphocytes expressing KLRG1 and CD45RO was greater in O than Y, whereas Y had a greater expression of CD45RA and CD62L than O. The percentage of all CD3+/CD8+ and CD3+/CD4+ T-lymphocytes expressing KLRG1 and CD57 increased after exercise, but the magnitude of change was not age-dependent. In conclusion, there is a greater proportion of senescent CD3+/CD8+ T-lymphocytes in the blood of older adults compared to young at rest and immediately after exhaustive exercise, indicating that the greater frequency of KLRG1+/CD8+ T-lymphocytes in older humans is ubiquitous and not localised to the peripheral blood.

Citation

Simpson, R. J., Cosgrove, C., Ingram, L. A., Florida-James, G. D., Whyte, G. P., Pircher, H., & Guy, K. (2008). Senescent T-lymphocytes are mobilised into the peripheral blood compartment in young and older humans after exhaustive exercise. Brain, Behavior, and Immunity, 22(4), 544-551. https://doi.org/10.1016/j.bbi.2007.11.002

• Journal Article Type: Article
• Acceptance Date: Nov 16, 2007
• Online Publication Date: Mar 4, 2008
• Publication Date: 2008-05
• Deposit Date: Aug 1, 2016
• Journal: Brain, Behavior, and Immunity
• Print ISSN: 0889-1591
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 4
• Pages: 544-551
• DOI: https://doi.org/10.1016/j.bbi.2007.11.002
• Keywords: KLRG1, CD57, CD28, Lymphocyte proliferation, flow cytometry, age,
• Public URL: http://researchrepository.napier.ac.uk/Output/321090