Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes

Harvey, Robert J.; St�hmer, Thorsten; van Minnen, Jan; Darlison, Mark G.

Abstract

We report the isolation of molluscan (Lymnaea stagnalis) partial complementary DNAs (cDNAs) that encode two new putative glutamate-gated cation-channel polypeptides. These proteins, which we have named Lym-eGluR4 and Lym-eGluR5, exhibit 56% identity to each other, and strong similarity (56% to 61%, and 56% to 68% identity, respectively) to the corresponding portions of vertebrate and invertebrate α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-selective (or AMPA-like) glutamate receptor (GluR) subunits. Using in situ hybridisation, we have shown that the Lym-eGluR4 and Lym-eGluR5 genes are both transcribed in all 11 central ganglia of the molluscan nervous system; however, their patterns of expression are quite different. Interestingly, the Lym-eGluR5 mRNA is present in an identifiable neuron (RPeD1), within the right pedal ganglion, that has recently been shown to possess a GluR that can be readily activated by L-glutamate, quisqualic acid and kainic acid. © 1997 John Wiley & Sons, Ltd.

Citation

Harvey, R. J., Stühmer, T., van Minnen, J., & Darlison, M. G. (1997). Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes. Neuroscience Research Communications, 20(1), 31-40. https://doi.org/10.1002/%28SICI%291520-6769%28199701%2920%3A13.0.CO%3B2-6

• Journal Article Type: Article
• Acceptance Date: Oct 25, 1996
• Publication Date: 1997-01
• Deposit Date: Jul 27, 2016
• Journal: Neuroscience Research Communications
• Print ISSN: 0893-6609
• Electronic ISSN: 1520-6769
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 20
• Issue: 1
• Pages: 31-40
• DOI: https://doi.org/10.1002/%28SICI%291520-6769%28199701%2920%3A13.0.CO%3B2-6
• Keywords: complementary DNA cloning; gene family; glutamate-gated cation channel; glutamate receptor; in situ hybridisation; mollusc (Lymnaea stagnalis)
• Public URL: http://researchrepository.napier.ac.uk/Output/317718
• Publisher URL: http://dx.doi.org/10.1002/(SICI)1520-6769(199701)20:13.0.CO;2-6