Growth hormone and amino acid supply interact synergistically to control insulin-like growth factor-I production and gene expression in cultured ovine hepatocytes.

Wheelhouse, Nicholas M; Stubbs, A K; Lomax, M A; MacRae, J C

doi:10.1677/joe.0.1630353

Growth hormone and amino acid supply interact synergistically to control insulin-like growth factor-I production and gene expression in cultured ovine hepatocytes.

Wheelhouse, Nicholas M; Stubbs, A K; Lomax, M A; MacRae, J C

Authors

Prof Nick Wheelhouse N.Wheelhouse@napier.ac.uk
Professor

A K Stubbs

M A Lomax

J C MacRae

Abstract

Many of the anabolic effects of growth hormone (GH) are indirect, occurring through GH-stimulated production of insulin-like growth factor-I (IGF-I) by the liver. As well as being regulated by GH, plasma IGF-I concentrations have been demonstrated to depend upon the level of dietary protein intake, with low protein diets being associated with reduced circulatory IGF-I levels. This inhibitory effect cannot be reversed by GH injection, suggesting that liver sensitivity to GH becomes impaired. To investigate the mechanisms through which protein supply affects GH sensitivity, primary cultures of ovine hepatocytes were grown in defined media, containing various proportions (0.2, 1.0 and 5.0) of jugular amino acid concentrations in fed sheep. Production of IGF-I by these cells was measured after 24 and 48 h in culture by radioimmunoassay. In the first 24-h period basal IGF-I production was the same in all defined media, and GH caused an approximately 2-fold increase in IGF-I release in cells grown in 1.0xor 5.0xamino acid media (P

Citation

Wheelhouse, N. M., Stubbs, A. K., Lomax, M. A., & MacRae, J. C. (1999). Growth hormone and amino acid supply interact synergistically to control insulin-like growth factor-I production and gene expression in cultured ovine hepatocytes. Journal of Endocrinology, 163(2), 353-361. https://doi.org/10.1677/joe.0.1630353

Journal Article Type	Article
Online Publication Date	Nov 1, 1999
Publication Date	Nov 1, 1999
Deposit Date	Jul 21, 2016
Journal	Journal of Endocrinology
Print ISSN	0022-0795
Electronic ISSN	1479-6805
Publisher	BioScientifica
Peer Reviewed	Peer Reviewed
Volume	163
Issue	2
Pages	353-361
DOI	https://doi.org/10.1677/joe.0.1630353
Keywords	Ovine, amino acid, growth hormone,
Public URL	http://researchrepository.napier.ac.uk/Output/304759
Related Public URLs	http://joe.endocrinology-journals.org/content/163/2/353.full.pdf