An enduring in vitro wound healing phase recipient by bioactive glass-graphene oxide nanocomposites

Nandhakumar, Manjubaashini; Thangaian, Daniel Thangadurai; Sundaram, Senthilarasu; Roy, Anurag; Subramanian, Balakumar

doi:10.1038/s41598-022-20575-z

Authors

Manjubaashini Nandhakumar

Daniel Thangadurai Thangaian

Dr Senthilarasu Sundaram S.Sundaram@napier.ac.uk
Associate Professor

Anurag Roy

Balakumar Subramanian

Abstract

Bioactive glass (BG) is an interesting topic in soft tissue engineering because of its biocompatibility and bonding potential to increase fibroblast cell proliferation, synthesize growth factors, and stimulate granulation tissue development. The proposed BG with and without sodium (Na), prepared by the sol–gel method, is employed in wound healing studies. The BG/graphene oxide (GO) and BG (Na-free)/GO nanocomposites were investigated against fibroblast L929 cells in vitro; the 45S5 BG nanocomposites exhibited desired cell viability (80%), cell proliferation (30%), cell migration (25%), metabolic activity, and wound contraction due to extracellular matrix (ECM) production and enhanced protein release by fibroblast cells. Additionally, the antioxidant assays for BG, BG (Na-free), GO, and BG/GO, BG (Na-free)/GO were evaluated for effective wound healing properties. The results showed decreased inflammation sites in the wound area, assessed by the (2,2-diphenyl-1-picryl-hydrazyl-hydrate) (DPPH) assay with ~ 80% radical scavenging activity, confirming their anti-inflammatory and improved wound healing properties.

Citation

Nandhakumar, M., Thangaian, D. T., Sundaram, S., Roy, A., & Subramanian, B. (2022). An enduring in vitro wound healing phase recipient by bioactive glass-graphene oxide nanocomposites. Scientific Reports, 12(1), Article 16162. https://doi.org/10.1038/s41598-022-20575-z

Journal Article Type	Article
Acceptance Date	Sep 15, 2022
Online Publication Date	Sep 28, 2022
Publication Date	2022
Deposit Date	Oct 5, 2022
Publicly Available Date	Oct 5, 2022
Journal	Scientific Reports
Publisher	Nature Publishing Group
Peer Reviewed	Peer Reviewed
Volume	12
Issue	1
Article Number	16162
DOI	https://doi.org/10.1038/s41598-022-20575-z
Public URL	http://researchrepository.napier.ac.uk/Output/2926522

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