In vitro transcription of Tomato spotted wilt virus is independent of translation

van Knippenberg, I.; Goldbach, R.; Kormelink, R.

Abstract

Ongoing transcription in vitro of Tomato spotted wilt virus (TSWV) has previously been demonstrated to require the presence of reticulocyte lysate. This dependence was further investigated by testing the occurrence of transcription in the presence of two translation inhibitors: edeine, an inhibitor that still allows scanning of nascent mRNAs by the 40S ribosomal subunit, and cycloheximide, an inhibitor that completely blocks translation including ribosome scanning. Neither of these inhibitors blocked TSWV transcription initiation or elongation in vitro, as demonstrated by de novo-synthesized viral mRNAs with globin mRNA-derived leader sequences, suggesting that TSWV transcription in vitro requires the presence of (a component within) reticulocyte lysate, rather than a viral protein resulting from translation.

Citation

van Knippenberg, I., Goldbach, R., & Kormelink, R. (2004). In vitro transcription of Tomato spotted wilt virus is independent of translation. Journal of General Virology, 85(5), https://doi.org/10.1099/vir.0.19767-0

• Journal Article Type: Article
• Publication Date: 2004-05
• Deposit Date: Jul 19, 2023
• Journal: The Journal of general virology
• Print ISSN: 0022-1317
• Electronic ISSN: 1465-2099
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 85
• Issue: 5
• DOI: https://doi.org/10.1099/vir.0.19767-0