Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif

van Knippenberg, I.; Goldbach, R.; Kormelink, R.

Abstract

The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3′-end of the intergenic hairpin, i.e. position 1568–1574 for N and position 1852–1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3′-end. The potential involvement of the 3′-end structure in transcription termination is discussed.

Citation

van Knippenberg, I., Goldbach, R., & Kormelink, R. (2005). Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif. Virus Research, 110(1-2), 125-131. https://doi.org/10.1016/j.virusres.2005.01.012

• Journal Article Type: Article
• Acceptance Date: Jan 22, 2005
• Online Publication Date: Mar 11, 2005
• Publication Date: 2005-06
• Deposit Date: Jul 19, 2023
• Journal: Virus research
• Print ISSN: 0168-1702
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 110
• Issue: 1-2
• Pages: 125-131
• DOI: https://doi.org/10.1016/j.virusres.2005.01.012