G. Blakqori
Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation
Blakqori, G.; van Knippenberg, I.; Elliott, R.M.
Abstract
The mRNAs of Bunyamwera virus (BUNV), the prototype of the Bunyaviridae family, possess a 5′ cap structure but lack a 3′ poly(A) tail, a common feature of eukaryotic mRNAs that greatly enhances translation efficiency. Viral mRNAs also contain untranslated regions (UTRs) that flank the coding sequence. Using model virus-like mRNAs that harbor the Renilla luciferase reporter gene, we found that the 3′ UTR of the BUNV small-segment mRNA mediated efficient translation in the absence of a poly(A) tail. Viral UTRs did not increase RNA stability, and polyadenylation did not significantly enhance reporter activity. Translation of virus-like mRNAs in transfected cells was unaffected by knockdown of poly(A)-binding protein (PABP) but was markedly reduced by depletion of eukaryotic initiation factor 4G, suggesting a PABP-independent process for translation initiation. In BUNV-infected cells, translation of polyadenylated but not virus-like mRNAs was inhibited. Furthermore, we demonstrate that the viral nucleocapsid protein binds to, and colocalizes with, PABP in the cytoplasm early in infection, followed by nuclear retention of PABP. Our results suggest that BUNV corrupts PABP function in order to inhibit translation of polyadenylated cellular mRNAs while its own mRNAs are translated in a PABP-independent process.
Citation
Blakqori, G., van Knippenberg, I., & Elliott, R. (2009). Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A) tail-independent translation. Journal of Virology, 83(8), https://doi.org/10.1128/jvi.02201-08
Journal Article Type | Article |
---|---|
Publication Date | 2009-04 |
Deposit Date | Jul 19, 2023 |
Journal | Journal of virology |
Print ISSN | 0022-538X |
Electronic ISSN | 1098-5514 |
Publisher | American Society for Microbiology |
Peer Reviewed | Peer Reviewed |
Volume | 83 |
Issue | 8 |
DOI | https://doi.org/10.1128/jvi.02201-08 |
You might also like
Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection
(2020)
Journal Article
Downloadable Citations
About Edinburgh Napier Research Repository
Administrator e-mail: repository@napier.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search